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The ADP receptor P2Y<inf>1 </inf>is necessary for normal thermal sensitivity in cutaneous polymodal nociceptors

Molliver, DC and Rau, KK and McIlwrath, SL and Jankowski, MP and Koerber, HR (2011) The ADP receptor P2Y<inf>1 </inf>is necessary for normal thermal sensitivity in cutaneous polymodal nociceptors. Molecular Pain, 7.

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Abstract

Background: P2Y1 is a member of the P2Y family of G protein-coupled nucleotide receptors expressed in peripheral sensory neurons. Using ratiometric calcium imaging of isolated dorsal root ganglion neurons, we found that the majority of neurons responding to adenosine diphosphate, the preferred endogenous ligand, bound the lectin IB4 and expressed the ATP-gated ion channel P2X3. These neurons represent the majority of epidermal afferents in hairy skin, and are predominantly C-fiber polymodal nociceptors (CPMs), responding to mechanical stimulation, heat and in some cases cold.Results: To characterize the function of P2Y1 in cutaneous afferents, intracellular recordings from sensory neuron somata were made using an ex vivo preparation in which the hindlimb skin, saphenous nerve, DRG and spinal cord were dissected in continuum, and cutaneous receptive fields characterized using digitally-controlled mechanical and thermal stimuli in male wild type mice. In P2Y1-/- mice, CPMs showed a striking increase in mean heat threshold and a decrease in mean peak firing rate during a thermal ramp from 31-52°C. A similar change in mean cold threshold was also observed. Interestingly, mechanical testing of CPMs revealed no significant differences between P2Y1-/- and WT mice.Conclusions: These results strongly suggest that P2Y1 is required for normal thermal signaling in cutaneous sensory afferents. Furthermore, they suggest that nucleotides released from peripheral tissues play a critical role in the transduction of thermal stimuli in some fiber types. © 2011 Molliver et al; licensee BioMed Central Ltd.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Molliver, DCdcm12@pitt.eduDCM12
Rau, KK
McIlwrath, SL
Jankowski, MP
Koerber, HRrkoerber@pitt.eduRKOERBER
Date: 10 February 2011
Date Type: Publication
Journal or Publication Title: Molecular Pain
Volume: 7
DOI or Unique Handle: 10.1186/1744-8069-7-13
Schools and Programs: School of Medicine > Medicine
School of Medicine > Neurobiology
Refereed: Yes
Date Deposited: 17 Nov 2016 19:59
Last Modified: 04 Jan 2020 16:55
URI: http://d-scholarship.pitt.edu/id/eprint/30182

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