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Temporal reliability of cytokines and growth factors in EDTA plasma

Clendenen, TV and Arslan, AA and Lokshin, AE and Idahl, A and Hallmans, G and Koenig, KL and Marrangoni, AM and Nolen, BM and Ohlson, N and Zeleniuch-Jacquotte, A and Lundin, E (2010) Temporal reliability of cytokines and growth factors in EDTA plasma. BMC Research Notes, 3.

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Background. Cytokines are involved in the development of chronic diseases, including cancer. It is important to evaluate the temporal reproducibility of cytokines in plasma prior to conducting epidemiologic studies utilizing these markers. Findings. We assessed the temporal reliability of CRP, 22 cytokines and their soluble receptors (IL-1α, ILβ-1, IL-1RA, IL-2, sIL-2R, IL-4, IL-5, IL-6, sIL-6R, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-17, TNFα, sTNF-R1, sTNF-R2, IFNα, IFNγ) and eight growth factors (GM-CSF, EGF, bFGF, G-CSF, HGF, VEGF, EGFR, ErbB2) in repeated EDTA plasma samples collected an average of two years apart from 18 healthy women (age range: 42-62) enrolled in a prospective cohort study. We also estimated the correlation between serum and plasma biomarker levels using 18 paired clinical samples from postmenopausal women (age range: 75-86). Twenty-six assays were able to detect their analytes in at least 70% of samples. Of those 26 assays, we observed moderate to high intra-class correlation coefficients (ICCs)(ranging from 0.53-0.89) for 22 assays, and low ICCs (0-0.47) for four assays. Serum and plasma levels were highly correlated (r > 0.6) for most markers, except for seven assays (r < 0.5). Conclusions. For 22 of the 31 biomarkers, a single plasma measurement is a reliable estimate of a woman's average level over a two-year period. © 2010 Clendenen et al; licensee BioMed Central Ltd.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Clendenen, TV
Arslan, AA
Lokshin, AElokshina@pitt.eduLOKSHINA
Idahl, A
Hallmans, G
Koenig, KL
Marrangoni, AM
Nolen, BMbmn9@pitt.eduBMN9
Ohlson, N
Zeleniuch-Jacquotte, A
Lundin, E
Centers: Other Centers, Institutes, Offices, or Units > Pittsburgh Cancer Institute
Date: 16 November 2010
Date Type: Publication
Journal or Publication Title: BMC Research Notes
Volume: 3
DOI or Unique Handle: 10.1186/1756-0500-3-302
Schools and Programs: School of Medicine > Medicine
School of Medicine > Obstetrics, Gynecology, and Reproductive Sciences
School of Medicine > Pathology
Refereed: Yes
Date Deposited: 16 Nov 2016 16:06
Last Modified: 04 Feb 2019 15:58


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