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Comparison of estrogens and estrogen metabolites in human breast tissue and urine

Taioli, E and Im, A and Xu, X and Veenstra, TD and Ahrendt, G and Garte, S (2010) Comparison of estrogens and estrogen metabolites in human breast tissue and urine. Reproductive Biology and Endocrinology, 8.

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Background: An important aspect of the link between estrogen and breast cancer is whether urinary estrogen levels are representative of the intra-tissue levels of bioavailable estrogens.Methods: This study compares 15 estrogen and estrogen metabolite levels in breast tissue and urine of 9 women with primary breast cancer using a quantitative liquid chromatography-mass spectrometry method.Results: The average levels of estrogens (estrone, 17 beta-estradiol) were significantly higher in breast tissue than in urine. Both the 2 and the 16-hydroxylation pathways were less represented in breast tissue than urine; no components of the 4-hydroxypathway were detected in breast tissue, while 4-hydroxyestrone was measured in urine. However, the 2/16 ratio was similar in urine and breast tissue. Women carrying the variant CYP1B1 genotype (Leu/Val and Val/Val) showed significantly lower overall estrogen metabolite, estrogen, and 16-hydroxylation pathway levels in breast tissue in comparison to women carrying the wild type genotype. No effect of the CYP1B1 polymorphism was observed in urinary metabolites.Conclusions: The urinary 2/16 ratio seems a good approximation of the ratio observed in breast tissue. Metabolic genes may have an important role in the estrogen metabolism locally in tissues where the gene is expressed, a role that is not readily observable when urinary measurements are performed. © 2010 Taioli et al; licensee BioMed Central Ltd.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Taioli, E
Im, Aimannie@pitt.eduIMANNIE
Xu, X
Veenstra, TD
Ahrendt, Ggea11@pitt.eduGEA11
Garte, S
Date: 2 August 2010
Date Type: Publication
Journal or Publication Title: Reproductive Biology and Endocrinology
Volume: 8
DOI or Unique Handle: 10.1186/1477-7827-8-93
Schools and Programs: School of Public Health > Public Health Genetics
School of Medicine > Surgery
Refereed: Yes
Date Deposited: 14 Nov 2016 16:51
Last Modified: 29 Jan 2019 15:55


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