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A porcine ex vivo model of pigmentary glaucoma

Dang, Yalong and Waxman, Susannah and Wang, Chao and Loewen, Ralista T and Sun, Ming and Loewen, Nils A A porcine ex vivo model of pigmentary glaucoma.

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<jats:title>Abstract</jats:title><jats:p>Pigment dispersion syndrome can lead to pigmentary glaucoma (PG), a poorly understood condition of younger, myopic eyes with fluctuating, high intraocular pressure (IOP). The absence of a model similar in size and behavior to human eyes has made it difficult to investigate its pathogenesis. Here, we present a porcine ex vivo model that recreates the features of PG including intraocular hypertension, pigment accumulation in the trabecular meshwork and relative failure of phagocytosis. In<jats:italic>in vitro</jats:italic>monolayer cultures as well as in<jats:italic>ex vivo</jats:italic>eye perfusion cultures, we found that the trabecular meshwork (TM) cells that regulate outflow, form actin stress fibers and have a decreased phagocytosis. Gene expression microarray and pathway analysis indicated key roles of RhoA in regulating the TM cytoskeleton, motility, and phagocytosis thereby providing new targets for PG therapy.</jats:p>


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Item Type: Article
CreatorsEmailPitt UsernameORCID
Dang, Yalongyad23@pitt.eduYAD23
Waxman, Susannah
Wang, Chao
Loewen, Ralista Trtl13@pitt.eduRTL13
Sun, Ming
Loewen, Nils ANAL58@pitt.eduNAL58
Publisher: Cold Spring Harbor Laboratory
DOI or Unique Handle: 10.1101/118448
Refereed: No
Date Deposited: 20 Mar 2017 16:00
Last Modified: 02 Aug 2020 13:58


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