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Trabecular meshwork failure in a model of pigmentary glaucoma

Dang, Yalong and Waxman, Susannah and Wang, Chao and Loewen, Ralitsa T. and Sun, Ming and Loewen, Nils A. (2017) Trabecular meshwork failure in a model of pigmentary glaucoma. (Submitted)

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Abstract

Pigment dispersion syndrome can lead to pigmentary glaucoma (PG), a poorly understood condition of younger, myopic eyes with fluctuating, high intraocular pressure (IOP). The absence of a model similar in size and behavior to human eyes has made it difficult to investigate the pathophysiology of IOP, pigment and phagocytosis. Here, we present a porcine ex vivo model that recreates the features of PG consisting of intraocular hypertension, pigment accumulation in the trabecular meshwork and failure of phagocytosis. In in vitro and ex vivo eye perfusion cultures we found that the trabecular meshwork (TM) cells that regulate outflow form of actin stress fibers, have a decreased phagocytosis and increased migration. Gene microarray and pathway analysis suggested key roles of RhoA and tight junctions in regulating the TM cytoskeleton, motility, and phagocytosis thereby providing new targets for future PG therapy.


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Details

Item Type: Article
Status: Submitted
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Dang, Yalongdangyalong@pitt.eduYAD23
Waxman, Susannah
Wang, Chao
Loewen, Ralitsa T.rtl13@pitt.eduRTL13
Sun, Ming
Loewen, Nils A.nal58@pitt.eduNAL58
Date: 17 March 2017
Date Type: Submission
DOI or Unique Handle: 10.1101/118448
Refereed: No
Funders: National Eye Institute
Article Type: Research Article
Date Deposited: 20 Mar 2017 16:00
Last Modified: 20 Mar 2017 16:00
URI: http://d-scholarship.pitt.edu/id/eprint/30990

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