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Glucose transporter expression in lung granulomas from Mycobacterium tuberculosis-infected monkeys

Liu, Hong (2017) Glucose transporter expression in lung granulomas from Mycobacterium tuberculosis-infected monkeys. Master's Thesis, University of Pittsburgh. (Unpublished)

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Abstract

TB remains to be one of the major threat to public health as it brings tremendous mobility and mortality in developing countries. Nevertheless, with the help from PET/CT imaging techniques, it provides a fast and sensitive way of diagnosing active TB and meanwhile, it proves to be valuable tool of studying TB inflammation and granuloma. Our study focused on glucose transporter expression in the granuloma tissues from infected monkeys as we believed their expression would be enhanced as a result of Warburg effect and increased inflammation. It can be the one of the major factors that drives uptake of 18F-FDG and give rise to high SUV from PET/CT results. However, our preliminary data did not fully support this hypothesis. Although initial RT-PCR data supported higher Glut1, Glut3, Glut8 and Glut14 expression from pooled granuloma tissue RNA, further quantification study from tissues with known SUV levels didn’t not show higher expression of glucose transporters in granuloma tissues in compare to normal tissues except Glut3. Individual comparison among glucose transporters in granuloma tissues showed lower expression relative to HPRT1 except Glut3 and most glucose transporter expression didn’t not show positive correlation with SUV levels. Although tissue staining experiment did confirmed higher expression of Glut1 and Glut3 in the granuloma tissues, MFI studies and statistical analysis didn’t not support expression of glucose transporters as a major contributor to the higher SUV levels. Therefore, there is not enough evidence to conclude glucose transporter expression in the granuloma tissues may be the main factor that drives FDG uptake and responsible for different SUV levels from PET/CT outcome.


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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Liu, Honghol38@pitt.eduhol38
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Thesis AdvisorJoshua, Mattilajmattila@pitt.edu
Committee MemberRobbie, Mailliardrbm19@pitt.edu
Committee MemberZeng, Dexing Zengzengd@upmc.edu
Date: 29 June 2017
Date Type: Publication
Defense Date: 20 April 2017
Approval Date: 29 June 2017
Submission Date: 3 April 2017
Access Restriction: 5 year -- Restrict access to University of Pittsburgh for a period of 5 years.
Number of Pages: 69
Institution: University of Pittsburgh
Schools and Programs: Graduate School of Public Health > Infectious Diseases and Microbiology
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: English
Date Deposited: 29 Jun 2017 23:18
Last Modified: 29 Jun 2017 23:18
URI: http://d-scholarship.pitt.edu/id/eprint/31573

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  • Glucose transporter expression in lung granulomas from Mycobacterium tuberculosis-infected monkeys. (deposited 29 Jun 2017 23:18) [Currently Displayed]

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