Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

Analysis of peripheral immune responses for the development of an encephalitis non-human primate animal model for new world alphaviruses

Salama, Noah (2017) Analysis of peripheral immune responses for the development of an encephalitis non-human primate animal model for new world alphaviruses. Master's Thesis, University of Pittsburgh. (Unpublished)

[img]
Preview
PDF
Submitted Version

Download (1MB) | Preview

Abstract

The New World alphaviruses eastern equine encephalitis virus (EEEV), Venezuelan equine encephalitis virus (VEEV), and western equine encephalitis virus (WEEV) are all mosquito-borne pathogens originating in North and South America. All three viruses are capable of causing severe illness in animals and humans, with extreme cases leading to encephalitis. Given the lack of commercially available vaccines or treatments, and previous research into these pathogens as potential bioterrorism weapons, all three are of great significance to public health. The aim of our laboratory was to develop a cynomolgus macaque model to study the unique neuropathology of each virus when delivered via an aerosol route, the most likely delivery route for a biological attack. To assess pathogenesis, peripheral blood mononuclear cells (PBMCs) were isolated from whole blood and analyzed by flow cytometry. Inflammation in the central nervous system (CNS) was also evaluated using a LEGENDplex bead based immunological array. Results indicate a substantial increase in the number of CCR2+ myeloid cells in circulation, a receptor important for homing and migration to infected tissue. In addition, inflammatory cytokines MCP-1 (CCL-2), IP-10, IL-6, and IL-8 were substantially upregulated in the CNS tissues and cerebrospinal fluid (CSF) of lethally infected animals. Collectively, this suggests homing and migration of peripheral immune cells to the CNS, and identifies potential markers for future longitudinal studies of alphavirus disease progression and severity.


Share

Citation/Export:
Social Networking:
Share |

Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Salama, NoahNAS197@pit.eduNAS197
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Thesis AdvisorHartman, Amyhatman2@pitt.edu
Committee MemberMailliard, Robbierbm19@pitt.edu
Committee MemberBarratt-Boyes, Simonsmbb@pitt.edu
Committee MemberKlimstra, Williamklimstra@pitt.edu
Date: 29 June 2017
Date Type: Publication
Defense Date: 19 April 2017
Approval Date: 29 June 2017
Submission Date: 27 April 2017
Access Restriction: 3 year -- Restrict access to University of Pittsburgh for a period of 3 years.
Number of Pages: 85
Institution: University of Pittsburgh
Schools and Programs: Graduate School of Public Health > Infectious Diseases and Microbiology
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: Viral encephalitis, alphavirus, animal model, immunology, neuroinflammation
Date Deposited: 29 Jun 2017 23:32
Last Modified: 01 May 2020 05:15
URI: http://d-scholarship.pitt.edu/id/eprint/31611

Metrics

Monthly Views for the past 3 years

Plum Analytics


Actions (login required)

View Item View Item