Deol, Harman
(2017)
Coronal Suture Morphology and Synostotic Progression in Rabbits with Delayed-Onset Craniosynostosis.
Master's Thesis, University of Pittsburgh.
(Unpublished)
This is the latest version of this item.
Abstract
Delayed-onset craniosynostosis has been reported in the human clinical literature and may represent part of the expanded phenotype of craniosynostosis. Delayed-onset synostosis (DOS) is also an integral part of the phenotypic spectrum in an inbred rabbit strain of craniosynostosis. The purpose of the present study was to qualitatively and quantitatively describe the perisynostotic events in the coronal sutures of rabbits with delay-onset coronal suture synostosis compared to age matched, wild-type control rabbits.
Coronal sutures from 80 rabbits (39 normal wild-type controls and 41 DOS) were collected for gross morphological and histological examination. Ages ranged from term (day 0) through 88 days of age. The sutures were harvested, formalin fixed, paraffin embedded, and stained with alizarin red for gross light microscopic analysis.
Qualitative results showed that by 25 days of age a dramatic thickening of the sutural ligament, an increase in the amount of bone in the osteogenic fronts, and an increase in the frequency of bony bridges between the frontal and parietal bones in rabbits with DOS compared to age matched, wild-type controls. No statistical significant differences in mean suture width or total suture area between the groups at the interdigitating zone were recorded at any age and cortical level (p> 0.05). At a region of the coronal suture closer to the midline, deemed the medial zone, a significant difference was found in mean suture width between groups at 88 day in age group at the ectocortical suture surface (p <.05); additionally, significant differences in mean suture area between the groups were recorded at age 25 and 88 (p <.05). A significant difference bony bridging frequency between the interdigitating zone and medial zone of DOS sutures was observed at the 45 day age group. The results of the 6X2 (age by group) two-way ANOVA indicate significant interaction at various cortical levels as well as total suture area at the interdigitating and medial zone (p < 0.05).
Results suggest that bony bridging and sutural ligament thickening occurs much earlier than coronal suture growth cessation in DOS rabbits and that these factors may limit subsequent sutural growth in an additive effect.
Share
| Citation/Export: |
|
| Social Networking: |
|
Details
| Item Type: |
University of Pittsburgh ETD
|
| Status: |
Unpublished |
| Creators/Authors: |
| Creators | Email | Pitt Username | ORCID  |
|---|
| Deol, Harman | hsd7@pitt.edu | hsd7@pitt.edu | |
|
| ETD Committee: |
|
| Date: |
18 April 2017 |
| Date Type: |
Completion |
| Defense Date: |
3 January 2017 |
| Approval Date: |
19 May 2017 |
| Submission Date: |
17 May 2017 |
| Access Restriction: |
No restriction; Release the ETD for access worldwide immediately. |
| Number of Pages: |
52 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
School of Dental Medicine > Dental Science |
| Degree: |
MS - Master of Science |
| Thesis Type: |
Master's Thesis |
| Refereed: |
No |
| Uncontrolled Keywords: |
Craniosynostosis Coronal Suture Morphology Delayed-onset |
| Date Deposited: |
19 May 2017 18:46 |
| Last Modified: |
19 May 2017 18:46 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/32042 |
Available Versions of this Item
-
Coronal Suture Morphology and Synostotic Progression in Rabbits with Delayed-Onset Craniosynostosis. (deposited 19 May 2017 18:46)
[Currently Displayed]
Metrics
Monthly Views for the past 3 years
Plum Analytics
Actions (login required)
 |
View Item |
![[feed]](/images/feed-icon-32x32.png){kind=icon}