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The Role of Prickle1 in the Development of the Pituitary Gland in the Beetlejuice Mouse

Shook, Corey (2017) The Role of Prickle1 in the Development of the Pituitary Gland in the Beetlejuice Mouse. Master's Thesis, University of Pittsburgh. (Unpublished)

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Abstract

Prickle1 is a protein that is a core component of the Wnt/Planar Cell Polarity pathway and is a key player in establishing cell polarity. To study the role of Prickle1, we are using the Beetlejuice mouse line. Beetlejuice mice have a missense mutation in Prickle1, and we used them to study the effects of Prickle1 in the development of the pituitary gland. We studied Beetlejuice mice at embryonic days 9.5 and 10.5 by utilizing hematoxylin and eosin (H&E) and immunofluorescence staining in order to visualize gland morphology and protein localization. Experiments utilizing dual immunofluorescence staining were performed using primary antibodies to Prickle1 and -catenin and to Pitx1 and ROR2. We found that Prickle1 is expressed in the cell membrane and in the nucleus of the wild type animals. However, in the Beetlejuice mutants, Prickle1 is scattered throughout the cytoplasm, and we did not see obvious cell membrane localization. We observed fewer Pitx1-positive cells in the Beetlejuice mutant, and the cells which are positive for Pitx1 are located in the edge of Rathke’s pouch. In the wild type, Pitx1 is localized to the nucleus. Beetlejuice mice have ROR2 dispersed throughout the cytoplasm, while in the wild type animals it is localized to the cell membrane. Based on our findings, we conclude that Prickle1 is required for cell polarization in normal pituitary gland development.


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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Shook, Coreycos36@pitt.educos36
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairSzabo-Rogers, Heatherhsrogers@pitt.eduhsrogers
Committee MemberWan, Yongyow11@pitt.eduyow11
Committee MemberBurnheimer, Johnjmb334@pitt.edujmb334
Committee MemberPetrone, Josephjfap@pitt.edujfap
Date: 26 June 2017
Date Type: Publication
Defense Date: 6 June 2017
Approval Date: 26 June 2017
Submission Date: 13 June 2017
Access Restriction: 3 year -- Restrict access to University of Pittsburgh for a period of 3 years.
Number of Pages: 32
Institution: University of Pittsburgh
Schools and Programs: School of Dental Medicine > Dental Science
Degree: MDS - Master of Dental Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: pituitary, Prickle1, Beetlejuice, anterior pituitary, mouse, Rathke's pouch
Date Deposited: 26 Jun 2017 13:51
Last Modified: 26 Jun 2017 13:51
URI: http://d-scholarship.pitt.edu/id/eprint/32445

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