Velásquez, Celestino
(2017)
VIRAL REGULATION OF CAP-DEPENDENT MESSENGER RNA TRANSLATION INHIBITOR 4E-BP1 DURING MITOSIS.
Doctoral Dissertation, University of Pittsburgh.
(Unpublished)
This is the latest version of this item.
Abstract
The work described in this dissertation started in 2013 and focuses on the activation of cap-dependent mRNA translation and regulation of the translation inhibitor protein 4E-BP1 during mitosis. By investigating the viral oncoprotein Merkel cell polyomavirus small T antigen (MCV sT), an alternative pathway for cap-dependent mRNA translation regulation through the CDK1 kinase was discovered. In addition, a novel phosphorylation site of 4E-BP1—Ser83—was identified in mitotic cells, and a polyclonal antibody against this phosphorylation site was generated. The phospho-Ser83 4E-BP1 antibody is a useful tool that can be used as a bona fide mitotic marker by various assays: immunoblotting, immunofluorescence, and flow cytometry. Overall, this work provokes a reassessment of protein synthesis regulation during cell division, hints at additional undescribed functions for 4E-BP1, and suggests that certain mRNAs translated in this phase of the cell cycle may contribute to cell proliferation and tumorigenesis.
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Details
Item Type: |
University of Pittsburgh ETD
|
Status: |
Unpublished |
Creators/Authors: |
|
ETD Committee: |
|
Date: |
12 July 2017 |
Date Type: |
Publication |
Defense Date: |
12 June 2017 |
Approval Date: |
12 July 2017 |
Submission Date: |
14 June 2017 |
Access Restriction: |
No restriction; Release the ETD for access worldwide immediately. |
Number of Pages: |
177 |
Institution: |
University of Pittsburgh |
Schools and Programs: |
School of Medicine > Integrative Molecular Biology |
Degree: |
PhD - Doctor of Philosophy |
Thesis Type: |
Doctoral Dissertation |
Refereed: |
Yes |
Uncontrolled Keywords: |
Merkel cell, Merkel cell polyomavirus, small T antigen, tumor viruses, cancer, 4E-BP1, cyclin-dependent kinase 1, mammalian target of rapamycin, mitosis, cap-dependent mRNA translation |
Date Deposited: |
12 Jul 2017 16:01 |
Last Modified: |
12 Jul 2017 16:01 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/32714 |
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