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Polymeric Micropatterned Films: A Platform For Enhanced Mucoadhesion

Pitale, Yohann (2017) Polymeric Micropatterned Films: A Platform For Enhanced Mucoadhesion. Master's Thesis, University of Pittsburgh. (Unpublished)

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Abstract

Polymeric films have been established as effective mucoadhesive drug delivery systems. However, certain issues such as poor contact time with mucosal surfaces due to constant renewal of mucin need to be addressed. Current delivery systems are unable to prolong the presence of drug at the required site of action, leading to sub-optimal therapeutic activity. This work focused on designing novel micropatterned polymeric films to interact closely with the mucosa which can ultimately increase residence time of drug and reduce dosing frequency. Films of various polymer compositions were prepared using a polydimethylsiloxane mold with depressions of circle, triangle and square of specific dimensions (50, 100 and 200 um). They were characterized for their three-dimensional (3D) morphology, mechanical properties, contact angle and mucoadhesive strength. Doxycycline hyclate was chosen as model drug to load in micropatterned films and investigate their in vitro release profile in conditions mimicking periodontitis. Micropatterned films were also seeded with macrophages to determine immune response the films would generate. We were able to develop a diverse set of micropatterned films distinct in their physico-chemical properties. Micropatterns were able to significantly enhance mucoadhesion compared to plain/unpatterned films due to their higher surface area and surface roughness. Hydrophobicity offered by patterns and the presence of mucoadhesive polymer were crucial in increasing difficulty of detachment of film from mucosa. Difference in hydrophobicity of materials also governed the morphology of cells that adhered on patterned films. Dissolution studies revealed that use of pH-sensitive polymers can retard the release of DOX in disease conditions, which is essential for reducing dosing frequency and minimizing antibiotic resistance. Collectively, this work shows that 3D micropatterned films can be made using Generally Recognized as Safe (GRAS) polymers and help improve contact with mucosa for a prolonged period.


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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Pitale, Yohannyap6@pitt.eduyap6
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Thesis AdvisorSant, VinayakVIS45@pitt.edu
Committee MemberRohan, Lisalrohan@mwri.magee.edu
Committee MemberLi, Songsol4@pitt.edu
Date: 25 July 2017
Date Type: Publication
Defense Date: 26 June 2017
Approval Date: 25 July 2017
Submission Date: 24 July 2017
Access Restriction: 5 year -- Restrict access to University of Pittsburgh for a period of 5 years.
Number of Pages: 73
Institution: University of Pittsburgh
Schools and Programs: School of Pharmacy > Pharmaceutical Sciences
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: Micropatterns, mucoadhesion, pH-sensitive, sustained release
Date Deposited: 25 Jul 2017 18:05
Last Modified: 25 Jul 2017 18:05
URI: http://d-scholarship.pitt.edu/id/eprint/32795

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