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On the use of ultracentrifugal devices for routine sample preparation in biomolecular magic-angle-spinning NMR

Mandal, A and Boatz, JC and Wheeler, TB and van der Wel, PCA (2017) On the use of ultracentrifugal devices for routine sample preparation in biomolecular magic-angle-spinning NMR. Journal of Biomolecular NMR, 67 (3). 165 - 178. ISSN 0925-2738

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Abstract

© 2017, Springer Science+Business Media Dordrecht. A number of recent advances in the field of magic-angle-spinning (MAS) solid-state NMR have enabled its application to a range of biological systems of ever increasing complexity. To retain biological relevance, these samples are increasingly studied in a hydrated state. At the same time, experimental feasibility requires the sample preparation process to attain a high sample concentration within the final MAS rotor. We discuss these considerations, and how they have led to a number of different approaches to MAS NMR sample preparation. We describe our experience of how custom-made (or commercially available) ultracentrifugal devices can facilitate a simple, fast and reliable sample preparation process. A number of groups have since adopted such tools, in some cases to prepare samples for sedimentation-style MAS NMR experiments. Here we argue for a more widespread adoption of their use for routine MAS NMR sample preparation.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Mandal, A
Boatz, JC
Wheeler, TB
van der Wel, PCApvdwel@pitt.eduPVDWEL0000-0002-5390-3321
Date: 1 March 2017
Date Type: Publication
Journal or Publication Title: Journal of Biomolecular NMR
Volume: 67
Number: 3
Page Range: 165 - 178
DOI or Unique Handle: 10.1007/s10858-017-0089-6
Schools and Programs: School of Medicine > Structural Biology
Refereed: Yes
ISSN: 0925-2738
Date Deposited: 28 Sep 2017 20:19
Last Modified: 02 Feb 2019 14:55
URI: http://d-scholarship.pitt.edu/id/eprint/33220

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