Ko, Ching-Chung
(2018)
EXPLORING MYCOBACTERIOPHAGE-ENCODED CYTOTOXINS.
Doctoral Dissertation, University of Pittsburgh.
(Unpublished)
Abstract
More than 1400 mycobacteriophage genomes have been completely sequenced to date. Comparative genomic analyses of the mycobacteriophages have revealed their highly diverse nature. Bioinformatics tools, however, were unable to assign functions to the majority of the mycobacteriophage-encoded genes, and thus the mycobacteriophage genomes provide a rich resource for insights into the biology of the phages and their hosts. This project investigates mycobacteriophage-encoded genes that impair the growth of the mycobacterial hosts. A total of 200 mycobacteriophage-encoded genes were screened for toxicity, identifying 21 toxic genes and 23 inhibitory genes. Based on PSI-BLAST and HHpred predictions, these toxic/inhibitory proteins have very different potential functions. At the same time, half of the toxic/inhibitory proteins have no predicted functions. Using co-immunoprecipitation assays, the interacting targets for two of the toxic proteins, Fruitloop gp52 and Wildcat gp13 were identified as Wag31 and MSMEG_1285, respectively. Identifying the basis of mycobacteriophage-encoded toxic protein action may provide insights into phage-host interaction, and into multiple processes that could be targeted for development of new anti-tuberculosis drugs.
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Details
| Item Type: |
University of Pittsburgh ETD
|
| Status: |
Unpublished |
| Creators/Authors: |
|
| ETD Committee: |
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| Date: |
31 January 2018 |
| Date Type: |
Publication |
| Defense Date: |
2 November 2017 |
| Approval Date: |
31 January 2018 |
| Submission Date: |
6 December 2017 |
| Access Restriction: |
5 year -- Restrict access to University of Pittsburgh for a period of 5 years. |
| Number of Pages: |
126 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
Dietrich School of Arts and Sciences > Biological Sciences |
| Degree: |
PhD - Doctor of Philosophy |
| Thesis Type: |
Doctoral Dissertation |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
Mycobacteriophage, Mycobacterium, Tuberculosis, Cytotoxin, Phage |
| Date Deposited: |
31 Jan 2018 17:35 |
| Last Modified: |
31 Jan 2023 06:15 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/33578 |
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