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Assessing the cytotoxicity of select antimicrobial peptides in the treatment of tuberculosis

Feldmiller, Joshua (2018) Assessing the cytotoxicity of select antimicrobial peptides in the treatment of tuberculosis. Master Essay, University of Pittsburgh.

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Abstract

Tuberculosis (TB) remains a leading cause of death despite the availability of drug treatments. Antibiotic resistance and genetic mutation has led to the development of both multi drug-resistant and extensively drug-resistant tuberculosis, further complicating successful intervention. Antimicrobial peptides (AMPs) present a novel alternative to standard antibiotic therapies. In this study, we used flow cytometry to measure the cytotoxicity of three different AMPs (A4S7, D8 and WLBU2) on three types of host cells associated with Tb infection. Both WLBU2 and A4S7 were associated with significant cell death (p < 0.05) at and above concentrations of 10μM, and the results for D8 fluctuated by cell type. The data obtained in this study will inform future replications in bacterial models, from which the plausibility of specific AMPs as treatments for Mycobacterium tuberculosis can be assessed and provide insights of clear public health significance.


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Details

Item Type: Other Thesis, Dissertation, or Long Paper (Master Essay)
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Feldmiller, Joshuajmf168@pitt.edujmf168
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
Committee ChairMattila, Joshuajmattila@pitt.edujmattilaUNSPECIFIED
Committee MemberDeslouches, Berthonytdesl19@pitt.edutdesl19UNSPECIFIED
Date: 2018
Date Type: Submission
Number of Pages: 35
Institution: University of Pittsburgh
Schools and Programs: Graduate School of Public Health > Infectious Diseases and Microbiology
Degree: MPH - Master of Public Health
Thesis Type: Master Essay
Refereed: Yes
Date Deposited: 20 Sep 2018 14:36
Last Modified: 20 Sep 2018 14:36
URI: http://d-scholarship.pitt.edu/id/eprint/34996

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