Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

Assessing the cytotoxicity of select antimicrobial peptides in the treatment of tuberculosis

Feldmiller, Joshua (2018) Assessing the cytotoxicity of select antimicrobial peptides in the treatment of tuberculosis. Master Essay, University of Pittsburgh.

[img]
Preview
PDF
Submitted Version

Download (222kB) | Preview

Abstract

Tuberculosis (TB) remains a leading cause of death despite the availability of drug treatments. Antibiotic resistance and genetic mutation has led to the development of both multi drug-resistant and extensively drug-resistant tuberculosis, further complicating successful intervention. Antimicrobial peptides (AMPs) present a novel alternative to standard antibiotic therapies. In this study, we used flow cytometry to measure the cytotoxicity of three different AMPs (A4S7, D8 and WLBU2) on three types of host cells associated with Tb infection. Both WLBU2 and A4S7 were associated with significant cell death (p < 0.05) at and above concentrations of 10μM, and the results for D8 fluctuated by cell type. The data obtained in this study will inform future replications in bacterial models, from which the plausibility of specific AMPs as treatments for Mycobacterium tuberculosis can be assessed and provide insights of clear public health significance.


Share

Citation/Export:
Social Networking:
Share |

Details

Item Type: Other Thesis, Dissertation, or Long Paper (Master Essay)
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Feldmiller, Joshuajmf168@pitt.edujmf168
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
Committee ChairMattila, Joshuajmattila@pitt.edujmattilaUNSPECIFIED
Committee MemberDeslouches, Berthonytdesl19@pitt.edutdesl19UNSPECIFIED
Date: 2018
Date Type: Submission
Number of Pages: 35
Institution: University of Pittsburgh
Schools and Programs: School of Public Health > Infectious Diseases and Microbiology
Degree: MPH - Master of Public Health
Thesis Type: Master Essay
Refereed: Yes
Date Deposited: 20 Sep 2018 14:36
Last Modified: 20 Sep 2018 14:36
URI: http://d-scholarship.pitt.edu/id/eprint/34996

Metrics

Monthly Views for the past 3 years

Plum Analytics


Actions (login required)

View Item View Item