Li, Wuyang (John)
(2018)
BEHAVIOR STUDY ON RATS WITH UNILATERAL SPLINT: IS SUDDEN CHANGE IN OCCLUSION RESPONSIBLE FOR TMD PAIN?
Master's Thesis, University of Pittsburgh.
(Unpublished)
This is the latest version of this item.
Abstract
Temporomandibular Joint (TMJ) Disorders (TMDs) affect 5-10% of the US population. One potential cause of TMD is a change in occlusion from trauma or surgery. In order to investigate whether a sudden change in occlusion is associated with the emergence of hypersensitivity in the TMJ area in adult rats, we performed perioral hypersensitivity assessment before and after splint placement on 12 male and 16 female Sprague Dawley rats with the orofacial pain assay. Rats were trained to access sucrose solution via a window in the side of the cage. Cumulative contact time (CT) with the sucrose sipper tube was determined for each 10 min training and subsequent testing session. For testing, 18-pin wire arrays were placed in the window to provide bi-lateral mechanical stimulation of the face when the sucrose solution was accessed. Baseline CTs were collected 3 times before the splint, and post-splint CTs were collected on different days depending on different batches. Splints consisted of dental resin poured to about 1 mm in thickness, which were applied unilaterally to the left maxillary molars. The experiment was conducted through 4 batches with 4 rats in the first batch and 8 rats in the other 3 batches. For the result, some of the rats in the splint group showed a decreasing trend for CT after splint placement while the others showed a transient decrease. For the Sham group, most rats in Batch 1 and 3 had very low CTs, while Batch 2 had high CTs before the splint but they decreased a lot after the splint placement. Batch 4 had high and stable CTs. No conclusion can be drawn from the current study because low CTs were seen in many rats before splint placement. For the future studies, more training could increase the baseline CT in order to detect the impact of splinting.
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Details
| Item Type: |
University of Pittsburgh ETD
|
| Status: |
Unpublished |
| Creators/Authors: |
| Creators | Email | Pitt Username | ORCID  |
|---|
| Li, Wuyang (John) | wul4@pitt.edu | wul4 | |
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| ETD Committee: |
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| Date: |
29 August 2018 |
| Date Type: |
Publication |
| Defense Date: |
1 August 2018 |
| Approval Date: |
29 August 2018 |
| Submission Date: |
9 August 2018 |
| Access Restriction: |
No restriction; Release the ETD for access worldwide immediately. |
| Number of Pages: |
60 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
School of Dental Medicine > Dental Science |
| Degree: |
MS - Master of Science |
| Thesis Type: |
Master's Thesis |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
Orofacial Pain Assay, TMJ |
| Date Deposited: |
29 Aug 2018 14:37 |
| Last Modified: |
29 Aug 2018 14:37 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/35249 |
Available Versions of this Item
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BEHAVIOR STUDY ON RATS WITH UNILATERAL SPLINT: IS SUDDEN CHANGE IN OCCLUSION RESPONSIBLE FOR TMD PAIN? (deposited 29 Aug 2018 14:37)
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