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Effects of Occlusal Change on the Histology of the Mandibular Condyle Cartilage of Rats

Liu, Xinyun (2018) Effects of Occlusal Change on the Histology of the Mandibular Condyle Cartilage of Rats. Master's Thesis, University of Pittsburgh. (Unpublished)

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We have previous data demonstrating that occlusal change in the rabbit results in degeneration of temporomandibular joint (TMJ) condyle, the major moving bone of the TMJ (Henderson, 2015). In the present study, we sought to determine whether comparable changes are observed in a rat model of occlusal change. Composite bite-raising splints (around 1mm) were applied to the left maxillary molars in 14-week-old male Sprague-Dawley rats for 4 weeks in the first batch (n=4) and 14-week-old female Sprague-Dawley rats 6 weeks in the second batch (n=4). Sham splint placement, where the splint was placed and then removed, served as the sham group (n=4) in both batches. At the end of splinting, all the rats were euthanized and histological analysis of the TMJ was carried out on both splinted and contralateral sides of the jaw with Hematoxylin & Eosin (H&E) for cellularity, Safranin-O staining for glycosaminoglycan(GAG) and Collagen II immunohistochemistry (IHC) staining. The presence of fibrous zone, proliferation zone, maturezone, hypertrophic zone served as primary tissue boundaries on the condyles ipsi- and contralateral to the splint placement. There did not seem to be a difference between the ipsi- and the contralateral sides after 4 and 6 weeks in sham group. While in the splinted group, there seem to be an increase in GAG on the ipsi- compared to the contralateral side after 4 weeks of splinting. Interestingly, after 6 weeks, the difference seemed less consistent compared to 4 weeks. Meanwhile, there appeared to be no GAG in sham group after 6 weeks. The Collagen II staining showed no significant difference in both sham and splinted group after 4 and 6 weeks. It will be important to further characterize the potential changes in other biochemical components of the joint tissue, time course of both the onset and recovery of the changes in the joint, and the extent to which the histological changes correlate with changes in joint sensitivity.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Liu, Xinyunxil182@pitt.eduxil182
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairAlmarza,
Committee MemberGold,
Committee MemberSzabo Rogers,
Committee MemberTaboas,
Date: 29 August 2018
Date Type: Publication
Defense Date: 2 August 2018
Approval Date: 29 August 2018
Submission Date: 23 August 2018
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Number of Pages: 46
Institution: University of Pittsburgh
Schools and Programs: School of Dental Medicine > Dental Science
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: Occlusal Change; Mandibular Condyle Cartilage
Date Deposited: 29 Aug 2018 14:37
Last Modified: 29 Aug 2018 14:37


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