King, Christina/D.
(2019)
Development and Applications of Enhanced Multiplexing to Better Understand Aging, Infection, and Alzheimer's Disease.
Doctoral Dissertation, University of Pittsburgh.
(Unpublished)
Abstract
Proteomics is the wide – scale study of proteins, in which the proteome of an organism is studied. Proteomics experiments obtain both qualitative and quantitative information by using a combination of analytical techniques including liquid chromatography, ion mobility, and mass spectrometry (MS). In quantitative experiments, relative protein amounts are determined to gain a better understanding of biological problems related to disease-state, kinetic changes, and effects of pharmaceutical products. In many cases, multiple samples (e.g. 10s to 100s) are analyzed to obtain statistically – significant results, which requires ample time (on the order of several days to weeks). To reduce sample analysis time and potential experimental error, multiplexing strategies have been developed. Samples are labeled metabolically, enzymatically, or chemically during sample preparation, pooled together, and analyzed simultaneously in the mass spectrometer.
In this body of work, multiplexing strategies have been applied to study aging and response to infection in C. elegans and the involvement of the periphery in Alzheimer’s disease. In addition, the development of analyzing multiplexed samples on Orbitrap MS platforms will be presented. These developments and applications contribute to science by providing insight to two conditions that affect the aging population.
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Details
| Item Type: |
University of Pittsburgh ETD
|
| Status: |
Unpublished |
| Creators/Authors: |
|
| ETD Committee: |
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| Date: |
30 January 2019 |
| Date Type: |
Publication |
| Defense Date: |
16 November 2018 |
| Approval Date: |
30 January 2019 |
| Submission Date: |
7 December 2018 |
| Access Restriction: |
1 year -- Restrict access to University of Pittsburgh for a period of 1 year. |
| Number of Pages: |
201 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
Dietrich School of Arts and Sciences > Chemistry |
| Degree: |
PhD - Doctor of Philosophy |
| Thesis Type: |
Doctoral Dissertation |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
proteomics, aging, cPILOT, multiplexing, Alzheimer's disease |
| Date Deposited: |
30 Jan 2019 23:30 |
| Last Modified: |
30 Jan 2020 06:15 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/35574 |
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