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Antibody localization in horse, rabbit, and goat antilymphocyte sera

Kashiwagi, N and Sherer, D and Townsend, CM and Jacobs, R and Ono, K and Kapur, B and Starzl, TE (1970) Antibody localization in horse, rabbit, and goat antilymphocyte sera. Surgery, 67 (5). 789 - 798. ISSN 0039-6060

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Abstract

The localization of antibodies was studied in rabbit, goat, and horse ALS raised by weekly immunization with canine or human spleen cells for 4 to 12 weeks. A combination of analytic techniques was used including column chromatography, electrophoresis, immunoelectrophoresis, determination of protein concentration, and measurement of antibody titers. In the rabbit and goat ALS, virtually all of the leukoagglutinins and lymphocytotoxins were in the easily separable IgG; accidentally induced thromboagglutinins were in the same location. In the rabbit hemagglutinins were found in both the IgG and IgM, whereas in the goat these were almost exclusively in the IgM. The antiwhite cell antibodies were most widely distributed in the horse. The cytotoxins were primarily in the IgG, but the leukoagglutinins were most heavily concentrated in the T-equine globulin which consists mostly of IgA. By differential ammonium sulfate precipitation of a horse antidoglymphocyte serum, fractions were prepared that were rich in IgG and IgA. Both were able to delay the rejection of canine renal homografts, the IgA-rich preparation to a somewhat greater degree. The findings in this study have been discussed in relation to the refining techniques that have been used for the production of globulin from heterologous ALS. © 1970.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Kashiwagi, N
Sherer, D
Townsend, CM
Jacobs, R
Ono, K
Kapur, B
Starzl, TEtes11@pitt.eduTES11
Centers: Other Centers, Institutes, or Units > Thomas E. Starzl Transplantation Institute
Date: 1 January 1970
Date Type: Publication
Journal or Publication Title: Surgery
Volume: 67
Number: 5
Page Range: 789 - 798
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0039-6060
Other ID: uls-drl:31735062124262, Starzl CV No. 196
Date Deposited: 08 Apr 2010 17:03
Last Modified: 05 Dec 2017 17:01
URI: http://d-scholarship.pitt.edu/id/eprint/3582

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