Mellett, Kaleigh
(2019)
Reltionship between variation the TP53 gene and patient outcomes following severe traumatic brain injury.
Undergraduate Thesis, University of Pittsburgh.
(Unpublished)
Abstract
Traumatic brain injury (TBI) is a leading cause of death and disability with millions of people living with long-term complications or disability related to TBI in the United States alone. This study examined the relationship between the p53 coding gene (TP53) and outcome variability following severe TBI. p53 has a known impact on neuronal apoptosis following TBI, which warrants investigation into TP53 genetic variability as a prognosticator for TBI outcomes (assessed using the Glasgow Outcome Scale, Neurobehavioral Scale, and Disability Rating Scale.) Participants (n = 429) were recruited from the UPMC Presbyterian Hospital with a Glasgow Coma Score £8 and were followed for 24 months. The single nucleotide polymorphism (SNP) rs1042522 was analyzed using restriction fragment length polymorphism (RFLP) and digested with BstuI restriction enzyme. Individuals with the CC genotype (Arginine homozygotes) were at risk for poorer outcomes following TBI at the 24-month point when compared to CG/GG variants. The findings provide preliminary evidence that p53 plays a role in recovery following TBI and, if replicated, may warrant investigation into p53 targeted therapies for risk allele carriers.
Share
| Citation/Export: |
|
| Social Networking: |
|
Details
| Item Type: |
University of Pittsburgh ETD
|
| Status: |
Unpublished |
| Creators/Authors: |
|
| ETD Committee: |
|
| Date: |
16 May 2019 |
| Date Type: |
Publication |
| Defense Date: |
1 April 2019 |
| Approval Date: |
16 May 2019 |
| Submission Date: |
28 March 2019 |
| Access Restriction: |
1 year -- Restrict access to University of Pittsburgh for a period of 1 year. |
| Number of Pages: |
27 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
School of Nursing > Nursing |
| Degree: |
BSN - Bachelor of Science in Nursing |
| Thesis Type: |
Undergraduate Thesis |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
Traumatic Brain Injury, p53, |
| Date Deposited: |
16 May 2020 05:00 |
| Last Modified: |
16 May 2020 05:15 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/36154 |
Metrics
Monthly Views for the past 3 years
Plum Analytics
Actions (login required)
 |
View Item |
![[feed]](/images/feed-icon-32x32.png){kind=icon}