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Relationship between variation in the TP53 gene and patient outcomes following severe traumatic brain injury

Mellett, Kaleigh (2019) Relationship between variation in the TP53 gene and patient outcomes following severe traumatic brain injury. Undergraduate Thesis, University of Pittsburgh. (Unpublished)

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Traumatic brain injury (TBI) is a leading cause of death and disability with millions of people living with long-term complications or disability related to TBI in the United States alone. This study examined the relationship between the p53 coding gene (TP53) and outcome variability following severe TBI. p53 has a known impact on neuronal apoptosis following TBI, which warrants investigation into TP53 genetic variability as a prognosticator for TBI outcomes (assessed using the Glasgow Outcome Scale, Neurobehavioral Scale, and Disability Rating Scale.) Participants (n = 429) were recruited from the UPMC Presbyterian Hospital with a Glasgow Coma Score 8 and were followed for 24 months. The single nucleotide polymorphism (SNP) rs1042522 was analyzed using restriction fragment length polymorphism (RFLP) and digested with BstuI restriction enzyme. Individuals with the CC genotype (Arginine homozygotes) were at risk for poorer outcomes following TBI at the 24-month point when compared to CG/GG variants. The findings provide preliminary evidence that p53 plays a role in recovery following TBI and, if replicated, may warrant investigation into p53 targeted therapies for risk allele carriers.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Mellett, Kaleighknm55@pitt.eduknm55
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Thesis AdvisorConley, Yvetteyconley@pitt.eduyconley
Committee MemberRen, Dianxudir8@pitt.edudir8
Committee MemberAlexander, Sheilasalexand@pitt.edusalexand
Committee MemberOsier,
Date: 16 April 2019
Date Type: Publication
Defense Date: 1 March 2019
Approval Date: 16 April 2019
Submission Date: 7 April 2019
Access Restriction: 1 year -- Restrict access to University of Pittsburgh for a period of 1 year.
Number of Pages: 27
Institution: University of Pittsburgh
Schools and Programs: David C. Frederick Honors College
School of Nursing > Nursing
Degree: BSN - Bachelor of Science in Nursing
Thesis Type: Undergraduate Thesis
Refereed: Yes
Uncontrolled Keywords: Traumatic brain injury, patient outcomes, single nucleotide polymorphism, p53
Date Deposited: 16 Apr 2019 19:22
Last Modified: 16 Apr 2020 05:15


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