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Does a functional SNP variance in interleukin-6 lead to increased physiology of delirium in ICU patients?

Kim, Janette (2019) Does a functional SNP variance in interleukin-6 lead to increased physiology of delirium in ICU patients? Undergraduate Thesis, University of Pittsburgh. (Unpublished)

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Abstract

Delirium is an acute alteration in mental status that impairs cognitive and physical functions. It presents with inattention, hallucinations, agitation, and overall cognitive and perceptual disturbances. Although older populations tend to experience delirium more frequently, the incidence of delirium in the intensive care unit (ICU) occurs in up to 80% of the population regardless of age. ICU delirium not only creates acute consequences for patients, but also may negatively affect patient outcomes in the long-term. Studies have associated ICU delirium with longer hospital stay, longer period of mechanical ventilation, increased incidence of cognitive impairment at discharge, and increased mortality risk regardless of age, sex, race, and severity of illness. Nonetheless, current delirium prevention, diagnosis, and treatment are inadequate due to a lack of understanding of its pathology.
This study aimed to explore a relationship between the biomarker interleukin-6 (IL-6) and delirium. Specifically, IL-6 has a polymorphism at position -174 of C/G that has shown to have effects on IL-6 production in different disease processes. This polymorphism was examined in relation to delirium for a relationship that may help explain delirium’s pathogenesis. The first specific aim was to explore if higher frequencies of a specific allele of IL-6 were present in patients who developed delirium. The second aim was to explore if a specific allele is also associated with more days of delirium. DNA was extracted from blood samples collected by a parent study and analyzed utilizing allelic discrimination in the Applied Biosystems ™ QuantStudio™ 3 Real-Time PCR System.
Data analysis included descriptive statistics, Pearson chi-square tests, univariate analyses, t-tests comparing genotype and days of delirium experienced, and a binary logistic regression model. These tests were utilized to find any association between IL-6 -174 genotypes (alleles C/G) with delirium and delirium positive days. We did not find statistically significant conclusions to support a specific genotype as being associated with delirium and delirium positive days. Additional studies that explore biomarkers in the role of delirium are needed to further investigate the biology and pathology of delirium to promote the development of proper prevention, diagnosis, and treatment in ICU patients and improve patient outcomes.


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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Kim, Janettejgk18@pitt.edujgk18
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairAlexander, Sheilasalexand@pitt.edu
Committee MemberConley, Yvetteyconley@pitt.edu
Committee MemberRen, Dianxudir8@pitt.edu
Committee MemberOlson, DaiWaiDaiwai.Olson@UTSouthwestern.edu
Date: 22 April 2019
Date Type: Publication
Defense Date: 7 March 2019
Approval Date: 22 April 2019
Submission Date: 15 April 2019
Access Restriction: 1 year -- Restrict access to University of Pittsburgh for a period of 1 year.
Number of Pages: 40
Institution: University of Pittsburgh
Schools and Programs: University Honors College
School of Nursing > Nursing
Degree: BSN - Bachelor of Science in Nursing
Thesis Type: Undergraduate Thesis
Refereed: Yes
Uncontrolled Keywords: interleukin-6, delirium, intensive care
Date Deposited: 22 Apr 2019 16:11
Last Modified: 22 Apr 2020 05:15
URI: http://d-scholarship.pitt.edu/id/eprint/36498

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