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Search for gut microbiota-mediated composition and influence in type 2 diabetes

Moore, Heather (2019) Search for gut microbiota-mediated composition and influence in type 2 diabetes. Undergraduate Thesis, University of Pittsburgh. (Unpublished)

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Type 2 diabetes mellitus (T2DM) has proven to be of utmost importance in clinical care, having been diagnosed in more than 29 million Americans in 2012 ("Statistics about Diabetes," 2012). The microbiome has a strong influence in the development of diabetes, but the relationship is not well understood. Methods: This current study utilized diabetic and nondiabetic porcine fecal samples, focusing on bacteria of interest from pre-existing studies to observe bacterial changes in the gut related to type 2 diabetes. We aimed to characterize the microbiota both compositionally and functionally. Relative abundance comparisons and retrospective analysis of the literature allowed a preliminary analysis of the alteration of the microbiota which may contribute to the consequent dysglycemia characteristic of type 2 diabetes. Results: Our results revealed, at the genus level, a significant decrease of intestinal bacterium Roseburia (p<0.0001) and Bacteroides (p=0.018) in the gut microbiome of diabetic compared to the non-diabetic porcine. Conclusion: This study revealed preliminary relationships between gut microbiome and diabetes in a porcine model. Roseburia, which was decreased in the diabetic microbiome, has been associated with anti-inflammatory mechanisms activated by the short chain fatty acid (SCFA) butyrate. This study found the pig to be a strong model for analysis. Further study may help with understanding potential mechanisms linking SCFA, the microbiome and diabetes. A more comprehensive analysis of taxonomic resolution of the gut microbiome to better define these complex interconnections is needed. A deeper understanding of the impact of this interaction on diabetes could help with the development of more targeted prevention mechanisms for at-risk populations and better management for diabetic patients.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Moore, HeatherHrm30@pitt.eduHrm30
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Thesis AdvisorYates,
Committee MemberConley,
Committee MemberFinegold,
Committee MemberIsmail,
Date: 20 May 2019
Date Type: Publication
Defense Date: 14 February 2019
Approval Date: 20 May 2019
Submission Date: 18 May 2019
Access Restriction: 1 year -- Restrict access to University of Pittsburgh for a period of 1 year.
Number of Pages: 35
Institution: University of Pittsburgh
Schools and Programs: David C. Frederick Honors College
School of Nursing > Nursing
Degree: BSN - Bachelor of Science in Nursing
Thesis Type: Undergraduate Thesis
Refereed: Yes
Uncontrolled Keywords: Microbiome, gut bacteria, type 2 diabetes mellitus
Date Deposited: 20 May 2019 12:54
Last Modified: 20 May 2020 05:15


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