Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

Renal sensory nerves increase sympathetic nerve activity and blood pressure in 2-Kidney 1-Clip hypertensive mice

Ong, Jason (2020) Renal sensory nerves increase sympathetic nerve activity and blood pressure in 2-Kidney 1-Clip hypertensive mice. Undergraduate Thesis, University of Pittsburgh. (Unpublished)

[img]
Preview
PDF
Download (660kB) | Preview

Abstract

Renal denervation lowers arterial blood pressure (ABP) in multiple clinical trials and some experimental models of hypertension. These antihypertensive effects have been attributed to the removal of renal afferent nerves. The purpose of the present study was to define the function, anatomy, and contribution of mouse renal sensory neurons to a renal-nerve dependent model of hypertension. First, electrical stimulation of mouse renal afferent nerves produced frequency-dependent increases in ABP that were eliminated by ganglionic blockade. Stimulus-triggered averaging revealed renal afferent stimulation significantly increased splanchnic, renal, and lumbar sympathetic nerve activity (SNA). Second, kidney injection of wheat germ agglutinin into male C57Bl6 mice (12-14 weeks, Jackson Laboratories) produced ipsilateral labeling in the T11-L2 dorsal root ganglia. Next, 2K1C hypertension was produced in male C57Bl6 mice (12-14 weeks, Jackson Laboratories) by placement of a 0.5mm length of PTFE tubing around the left renal artery. 2K1C mice displayed an elevated ABP measured via telemetry and greater fall in mean ABP to ganglionic blockade at day 14 or 21 vs day 0. Renal afferent discharge was significantly higher in 2K1C-clipped versus 2K1C-unclipped or sham kidneys. In addition, 2K1C-clipped versus 2K1C-unclipped or sham kidneys had lower renal mass and higher mRNA levels of several pro-inflammatory cytokines. Finally, both ipsilateral renal denervation (10% phenol) or selective denervation of renal afferent nerves (periaxonal application of 33 mM capsaicin) at time of clipping resulted in lower ABP of 2K1C mice at Days 14 or 21. These findings suggest mouse renal sensory neurons are activated to increase SNA and ABP in 2K1C hypertension.


Share

Citation/Export:
Social Networking:
Share |

Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Ong, Jasonjao60@pitt.edujao60
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairStocker, Seanstockers@pitt.edu
Committee MemberSesack, Susansesack@pitt.edu
Committee MemberSved, Alansved@pitt.edu
Committee MemberFink, Gregoryfinkg@msu.edu
Date: 1 May 2020
Date Type: Publication
Defense Date: 13 February 2020
Approval Date: 1 May 2020
Submission Date: 3 March 2020
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Number of Pages: 43
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Neuroscience
University Honors College
Degree: BPhil - Bachelor of Philosophy
Thesis Type: Undergraduate Thesis
Refereed: Yes
Uncontrolled Keywords: autonomic, blood pressure, hypertension, sensory, sympathetic
Date Deposited: 01 May 2020 18:49
Last Modified: 01 May 2020 18:49
URI: http://d-scholarship.pitt.edu/id/eprint/38297

Metrics

Monthly Views for the past 3 years

Plum Analytics


Actions (login required)

View Item View Item