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Development of Micropatterned, Mucoadhesive, Ocular Films for the Treatment of Diabetic Keratopathy

Vasudevan, Aishwarya (2020) Development of Micropatterned, Mucoadhesive, Ocular Films for the Treatment of Diabetic Keratopathy. Master's Thesis, University of Pittsburgh. (Unpublished)

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Abstract

According to the CDC report in 2017, approximately 9.4% of the US population suffers from diabetes. The ocular manifestations of diabetes include diabetic retinopathy, glaucoma, cataract, and ocular surface diseases. Research shows that around 47-64% of diabetic individuals suffer from dysfunctional corneal wound healing in the eye, known as diabetic keratopathy. The presence of free oxygen radicals, advanced glycation products, and the absence of mucins in the eyes of diabetic individuals disrupt the homeostasis required for epithelial wound healing. Current treatment for corneal wound healing is mostly symptomatic and includes administration of antibiotic drops, bandage lenses, artificial tears, and punctal plugs. However, these are symptom-based treatments, and there remains a need for a cause-based approach. Rebamipide eye drops are used in dry eye disease in Japan because of its ability to improve mucin secretion and maintain tear film stability.
Additionally, it has shown epithelial wound healing activity along with the ability to scavenge reactive oxygen species and reduce inflammation. In this study, we have fabricated micropatterned, mucoadhesive films containing rebamipide that have a sustained release of drug from the polymer matrix. We show that these thin films containing cellulose and Eudragit polymers form clear and distinct patterns of good resolution. Micropatterned films also show good tensile strength and flexibility. Cytotoxicity studies on corneal stem cells indicate that the 48%EPO+32%FS30D formulation has the least toxicity with a good sustained release of drug in 24 hours. We also demonstrate the higher mucoadhesive properties of micropatterned films as compared to plain, unpatterned films on the ocular mucosa. Finally, we show the preliminary development of an ex-vivo lacrimation model of the retention of films on the sclera using enucleated porcine eyeballs.


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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Vasudevan, Aishwaryavasudevan.aishwarya@outlook.comaiv5@pitt.edu0000-0002-4021-4949
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairSant, Vinayakvis45@pitt.eduvis45@pitt.edu
Committee MemberRohan, Lisarohanl@pitt.edurohanl@pitt.edu
Committee MemberLi, Songsol4@pitt.edusol4@pitt.edu
Date: 14 April 2020
Date Type: Publication
Defense Date: 1 April 2020
Approval Date: 14 April 2020
Submission Date: 13 April 2020
Access Restriction: 2 year -- Restrict access to University of Pittsburgh for a period of 2 years.
Number of Pages: 75
Institution: University of Pittsburgh
Schools and Programs: School of Pharmacy > Pharmaceutical Sciences
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: Diabetes, diabetic keratopathy, ocular surface diseases, micropatterns, mucins, Rebamipide, films
Date Deposited: 14 Apr 2020 16:52
Last Modified: 14 Apr 2020 16:52
URI: http://d-scholarship.pitt.edu/id/eprint/38620

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