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MMP2 and Aquaporin, Whole Saliva pH and Treatment Needs

Ahuja, Parul (2020) MMP2 and Aquaporin, Whole Saliva pH and Treatment Needs. Master's Thesis, University of Pittsburgh. (Unpublished)

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Objectives: Salivary pH ranges from 6.2 to 7.4 and is kept near neutrality. We hypothesized that within the normal physiological limits of the saliva some people tend to show more susceptibility/ resistance towards dental conditions. We also hypothesized that genetics play a role in controlling the saliva pH of the mouth and individuals within a certain range of this pH variation may show more extensive and customized dental treatment needs due to difference in their oral environment pertaining to differences in the genetic makeup of all individuals. This can be the explanation of why some individuals are more or less susceptible to dental diseases. We investigated the potential association between saliva pH and various treatment needs with MMP2 and the aquaporin locus.
Methods: Clinical data and saliva samples were obtained from the University of Pittsburgh Dental Registry and DNA Repository project. Analysis was performed by querying the medical and dental records of more than 6000 patients. A total of 189 patients were selected with almost equal number of males and females and assessed for their saliva pH values. pH was determined by the use of commercial strips. Some patients required stimulation to provide a saliva sample and it was done by the use of gum or candy to stimulate salivary secretion. Sex and ethnicity were also considered. Subjects were divided in two groups based on the pH of their saliva (above or below the mean pH of all subjects). Information was retrieved on positive/negative history for
hypertension, oral surgery treatment (as a surrogate for tooth loss), periodontitis, restorative treatment, and root canal therapy. Genetic association studies were also performed and the markers rs2241145, rs243865, rs243832, rs2285053, rs1163960 in MMP2 and the marker rs461872 in the aquaporin locus were genotyped using TaqMan chemistry and end-point analysis. Chi-square and Fisher’s exact tests were used in all comparisons with an alpha of 0.05.
Results: Out of the 189 subjects, 95 patients provided a saliva sample without the need for stimulation, 97 subjects were females, and 23 were African descendants. The mean pH of the saliva in all study participants was 7.16. We observed statistically significant differences in the distribution of saliva pH for the following genetic markers and phenotypes:
a. Periodontitis associated with rs11639960 and rs2285053.
b. Restorative treatment associated with rs243865, rs11639960, and rs2285053
c. Oral surgery treatment associated with rs11639960, rs2285053, rs243832, and rs461872
d. Root canal therapy associated with rs243865, rs11639960, rs11639960 and rs461872
e. Hypertension associated with rs243865 rs11639960, rs11639960, rs2285053, rs2241145 and rs461872
Conclusion: Our findings suggest that markers in MMP2 and the aquaporin locus associate with specific dental and systemic conditions within a particular range of the expected normal salivary pH. These findings could help us predict dental treatment needs of individuals considering genotyping of markers in MMP2 and the aquaporin locus.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Ahuja, Parulpaa46@pitt.edupaa460000-0002-4408-4117
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Thesis AdvisorVieira, Alexandre R./ ARVarv11@pitt.eduarv11
Committee ChairVieira, Adriana Modesto/ AMGSVams208@pitt.eduams208
Committee CoChairTaboas, Juan M/ JMTjmt106@pitt.edujmt106
Committee MemberMooney, Mark P/ MPMmpm4@pitt.edumpm4
Date: 22 May 2020
Date Type: Publication
Defense Date: 6 April 2020
Approval Date: 22 May 2020
Submission Date: 17 April 2020
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Number of Pages: 150
Institution: University of Pittsburgh
Schools and Programs: School of Dental Medicine > Dental Science
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: saliva pH , Genotyping, genetics, periodontitis, dental caries, hypertension, extraction, oral surgery, root canal therapy, MMP2, Aquaporin
Date Deposited: 22 May 2020 16:11
Last Modified: 22 May 2020 16:11


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