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Stem Cell and Tissue-Based Therapies for Male Infertility

David, Sherin (2020) Stem Cell and Tissue-Based Therapies for Male Infertility. Doctoral Dissertation, University of Pittsburgh. (Unpublished)

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Abstract

Infertility due to exposure to chemotherapy and radiation has a detrimental effect of the patient’s quality of life. A number of spermatogonial stem cell (SSC)-based methods are currently being developed for prepubertal male patients that cannot produce sperm for cryopreservation at the time of treatment. Spermatogonial stem cell culture and transplantation, and testicular tissue xenografting are technologies that have been proven to be successful in a number of mammalian species. Efforts to apply these technologies to human SSCs are currently underway. This dissertation describes work carried out to optimize methods for culturing human SSCs in vitro and to promote survival and maturation of cryopreserved and thawed prepubertal primate testicular xenografts in mice. We developed a multiparametric flow cytometry-based method to quantify human SSCs and used it to optimize the substrate and medium for culturing human SSCs. We also showed that human chorionic gonadotropin exposure promotes growth of both human and Rhesus xenografts and complete maturation of Rhesus xenografts. Additional studies need to be carried out to further develop both SSC culture and human xenografting technologies for application in the clinic for fertility preservation.


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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
David, Sherinshd52@pitt.edushd52
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairOrwig, Kylekorwig@mwri.magee.edu
Committee MemberTsang, Michaeltsang@pitt.edu
Committee MemberWalker, Williamwalkerw@pitt.edu
Committee MemberSoto-Gutierrez, Alejandroals208@pitt.edu
Committee MemberDuncan, Andrewduncana@pitt.edu
Date: 4 May 2020
Date Type: Publication
Defense Date: 5 February 2020
Approval Date: 4 May 2020
Submission Date: 23 April 2020
Access Restriction: 1 year -- Restrict access to University of Pittsburgh for a period of 1 year.
Number of Pages: 157
Institution: University of Pittsburgh
Schools and Programs: School of Medicine > Molecular Genetics and Developmental Biology
Degree: PhD - Doctor of Philosophy
Thesis Type: Doctoral Dissertation
Refereed: Yes
Uncontrolled Keywords: Spermatogonial stem cells, oncofertility
Date Deposited: 04 May 2020 11:33
Last Modified: 04 May 2020 11:33
URI: http://d-scholarship.pitt.edu/id/eprint/38779

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