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Rapid Divergence of Wing Volatile Profiles Between Subspecies of the Butterfly Pieris rapae (Lepidoptera: Pieridae)

McQueen, Eden W and Morehouse, Nathan I (2018) Rapid Divergence of Wing Volatile Profiles Between Subspecies of the Butterfly Pieris rapae (Lepidoptera: Pieridae). Journal of Insect Science, 18 (2). ISSN 1536-2442

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Complex signaling traits such as pheromone profiles can play an important role in the early stages of reproductive isolation between populations. These signals can diverge along multiple trait axes, and signal receivers are often sensitive to subtle differences in signal properties. In the Lepidoptera, prior research has highlighted that natural selection can drive rapid chemical signal divergence, for instance via mate recognition to maintain species boundaries. Much less is known about the occurrence of such changes for predominantly sexually selected chemical signals, such as those released by many male lepidopterans. We evaluated the divergence in male and female wing volatile profiles between two recently isolated subspecies of the pierid butterfly Pieris rapae Linnaeus (Lepidoptera: Pieridae): P. rapae rapae and P. rapae crucivora. In laboratory settings, these subspecies exhibit strong premating isolation, with females rejecting males of the opposite subspecies despite the fact that males direct equivalent courtship effort toward females of either subspecies. Using gas chromatography–mass spectrometry, we analyzed the volatile chemical profiles of individual males and females of each subspecies. We find that males of each subspecies differ in their wing volatile profiles, including quantitative differences in a male sex pheromone, ferrulactone. In contrast, female wing volatiles profiles have diverged significantly less. These sexspecific patterns suggest that male chemical profiles may play a role in the observed premating isolation between these two subspecies, providing support for future investigations of sexually selected chemical traits in population divergence.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
McQueen, Eden Wewo3@pitt.eduewo3
Morehouse, Nathan Inim@pitt.edunim
Date: 2018
Date Type: Publication
Journal or Publication Title: Journal of Insect Science
Volume: 18
Number: 2
Publisher: Oxford University Press
DOI or Unique Handle: 10.1093/jisesa/iey026
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
Uncontrolled Keywords: pheromones, speciation, allopatry, Lepidoptera
ISSN: 1536-2442
Official URL:
Funders: University of Pittsburgh
Article Type: Research Article
Date Deposited: 13 May 2020 15:26
Last Modified: 13 May 2020 15:26


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