Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

Projection Stereolithographic Fabrication of BMP-2 Gene-activated Matrix for Bone Tissue Engineering

Lin, Hang and Tang, Ying and Lozito, Thomas P. and Oyster, Nicholas and Kang, Robert B. and Fritch, Madalyn R. and Wang, Bing and Tuan, Rocky S. (2017) Projection Stereolithographic Fabrication of BMP-2 Gene-activated Matrix for Bone Tissue Engineering. Scientific Reports, 7 (1). ISSN 2045-2322

Published Version

Download (1MB) | Preview


Abstract Currently, sustained in vivo delivery of active bone morphogenetic protein-2 (BMP-2) protein to responsive target cells, such as bone marrow-derived mesenchymal stem cells (BMSCs), remains challenging. Ex vivo gene transfer method, while efficient, requires additional operation for cell culture and therefore, is not compatible with point-of-care treatment. In this study, two lentiviral gene constructs – (1) Lv-BMP/GFP, containing human BMP-2 and green fluorescent protein (GFP) gene (BMP group); or (2) Lv-GFP, containing GFP gene (GFP group) – were incorporated with human BMSCs into a solution of photocrosslinkable gelatin, which was then subjected to visible light-based projection stereolithographic printing to form a scaffold with desired architectures. Upon in vitro culture, compared to the GFP group, cells from BMP group showed >1,000-fold higher BMP-2 release, and the majority of them stained intensely for alkaline phosphatase activity. Real-time RT-PCR also showed dramatically increased expression of osteogenesis marker genes only in the BMP group. 3.5 months post-implantation into SCID mice, the micro-computed tomography imaging showed detectable mineralized areas only in the BMP group, which was restricted within the scaffolds. Alizarin red staining and immunohistochemistry of GFP and osteocalcin further indicated that the grafted hBMSCs, not host cells, contributed primarily to the newly formed bone.


Social Networking:
Share |


Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Lozito, Thomas P.
Oyster, Nicholas
Kang, Robert B.
Fritch, Madalyn R.
Tuan, Rocky
Date: 12 September 2017
Date Type: Publication
Journal or Publication Title: Scientific Reports
Volume: 7
Number: 1
Publisher: Nature Publishing Group
DOI or Unique Handle: 10.1038/s41598-017-11051-0
Schools and Programs: Swanson School of Engineering > Bioengineering
Refereed: Yes
ISSN: 2045-2322
Official URL:
Article Type: Research Article
Date Deposited: 12 May 2020 15:07
Last Modified: 12 May 2020 15:07


Monthly Views for the past 3 years

Plum Analytics

Actions (login required)

View Item View Item