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Functional characterization of infiltrating T lymphocytes in human hepatic allografts

Fung, JJ and Zeevi, A and Starzl, TE and Demetris, J and Iwatsuki, S and Duquesnoy, RJ (1986) Functional characterization of infiltrating T lymphocytes in human hepatic allografts. Human Immunology, 16 (2). 182 - 199. ISSN 0198-8859

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Abstract

We have employed recently developed techniques in T-cell culturing to study the nature and function of infiltrating hepatic allograft T cells. Using the rationale that intragraft T cells are activated during cell mediated damage to the allograft, we were able to show that these cells would propagate and remain functionally active in the presence of the T-cell growth factor, IL-2. In several instances, phenotyiic analysis of cells grown in this manner was very similar to that found within the graft. Both proliferative and cytotoxic responses could be detected from the cultured cell lines. The majority of the proliferative responses were donor-directed and immunogenetic analysis could define donor-directed HLA reactivity, to either class I or class II antigens, or both. Monoclonal anti-HLA antibodies inhibition profiles verified the apparent HLA reactivity. In a smaller percentage of cases, only IL-2 responsiveness could be detected, and no HLA reactivity could be determined. Cytotoxicity could be detected against both class I and class II antigens, however, those cells which demonstrated a greater magnitude of donor-directed cytotoxicity appeared to be directed against class I antigens. A significant correlation between donor-directed proliferation of biopsy cultured lymphocytes and cellular rejection was found. This model appears to be useful in delineating functions of the intragraft T-cell population during rejection. © 1986.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Fung, JJ
Zeevi, A
Starzl, TEtes11@pitt.eduTES11
Demetris, J
Iwatsuki, S
Duquesnoy, RJ
Centers: Other Centers, Institutes, or Units > Thomas E. Starzl Transplantation Institute
Date: 1 January 1986
Date Type: Publication
Journal or Publication Title: Human Immunology
Volume: 16
Number: 2
Page Range: 182 - 199
DOI or Unique Handle: 10.1016/0198-8859(86)90047-9
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0198-8859
Other ID: uls-drl:31735062116219, Starzl CV No. 662
Date Deposited: 08 Apr 2010 17:11
Last Modified: 04 Feb 2019 15:56
URI: http://d-scholarship.pitt.edu/id/eprint/4048

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