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Human Adenovirus in Children with Acute Gastroenteritis in the New Vaccine Surveillance Network (NVSN)

Kinzler, Amy (2021) Human Adenovirus in Children with Acute Gastroenteritis in the New Vaccine Surveillance Network (NVSN). Master's Thesis, University of Pittsburgh. (Unpublished)

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Acute gastroenteritis (AGE) is a leading global cause of pediatric morbidity and mortality. Human adenoviruses (HAdV) are a cause, but the burden is not fully defined. We sought to determine the prevalence and clinical features of HAdV F40/41 in pediatric patients at seven U.S. children’s hospitals. We enrolled children with AGE or age-matched healthy controls between 12/5/2016-11/30/2017 in the Centers for Disease Control and Prevention (CDC) New Vaccine Surveillance Network (NVSN). Seven NVSN sites enrolled a total of 4,831 subjects between inpatient, ED, and healthy controls. Demographic and clinical data and stool samples were prospectively collected. Stool samples were tested for HAdV using multiplex PCR. HAdV-positive samples from Pittsburgh and Kansas City sites were sequenced for genotyping. A total of 1,980 subjects were included in the study. In the ED and inpatient settings, 108 (16.7%) and 77 (10%) patients tested positive for HAdV, respectively, while only 19 (3.4%) healthy control patients tested positive. Among all subjects with AGE, the overall average age was 2.1 years old (SD ± 3.1y), but inpatients were older (3 + 4.1 years) compared to healthy controls (1.6 ± 2.3y) and ED patients (1.2 ± 1.7y). The most common symptoms of HAdV in both inpatient and ED settings were fever, diarrhea, and vomiting. HAdV+ children experienced significantly more diarrhea than HAdV- children in both inpatient and ED. Six genotypes were detected: B3 (2/120), C1 (5/120), C2 (2/120), C5 (1/120), F40 (4/120) with majority F41 (106/120). There was no difference in genotypes by site or clinical setting. HAdVs are a leading cause of AGE in young children, often resulting in hospitalization. The majority of cases were type F41, which has implications for vaccine development.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Kinzler, AmyAJK150@pitt.eduAJK150
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairMartinson, Jeremyjmartins@pitt.edujmartins
Committee MemberWilliam, Johnjvw@pitt.edujvw
Committee MemberChen, YueCHENY@pitt.educheny
Date: 12 May 2021
Date Type: Publication
Defense Date: 21 April 2021
Approval Date: 12 May 2021
Submission Date: 29 April 2021
Access Restriction: 1 year -- Restrict access to University of Pittsburgh for a period of 1 year.
Number of Pages: 32
Institution: University of Pittsburgh
Schools and Programs: School of Public Health > Infectious Diseases and Microbiology
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: N/A
Date Deposited: 13 May 2021 02:37
Last Modified: 12 May 2022 05:15


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