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Extraction and Partial Purification of a Hepatic Stimulatory Substance in Rats, Mice, and Dogs

Francavilla, A and Polimeno, L and Coetzee, M and Makowka, L and Rose, J and Van Thiel, DH (1987) Extraction and Partial Purification of a Hepatic Stimulatory Substance in Rats, Mice, and Dogs. Cancer Research, 47 (21). 5600 - 5605. ISSN 0008-5472

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Abstract

A factor has been isolated from weanling rat liver which stimulates in vivo hepatic DNA synthesis in a dose dependent manner when injected into 40% hepatectomized rats. The factor has been partially purified by successive steps, involving ethanol precipitation, ultrafiltration through an Amicon PM 30 membrane, and finally fast protein liquid chromatography, resulting in a 38,000-fold increase in specific activity over that in the original cytosol. The factor contains a few bands in the molecular weight range of 14,000-50,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Active fractions from fast protein liquid chromatography (F150), when injected into 40% hepatectomized rats, increased hepatic DNA synthesis 3-fold over the background stimulation due to the hepatectomy. The response was dose dependent over a range from 1.76 μg to 6.8 μg per 200-g (body weight) rat. Mitotic and labeling indexes confirmed that F150 stimulates both replicative DNA synthesis and cell proliferation. The factor is heat and neuraminidase resistant, trypsin sensitive, organ specific, but not species specific. © 1987, American Association for Cancer Research. All rights reserved.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Francavilla, A
Polimeno, L
Coetzee, M
Makowka, L
Rose, J
Van Thiel, DH
Centers: Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute
Date: 1 November 1987
Date Type: Publication
Journal or Publication Title: Cancer Research
Volume: 47
Number: 21
Page Range: 5600 - 5605
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0008-5472
Other ID: uls-drl:31735062119072, Starzl CV No. 713
Date Deposited: 08 Apr 2010 17:12
Last Modified: 23 Jan 2019 22:55
URI: http://d-scholarship.pitt.edu/id/eprint/4099

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