Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

The Antimicrobial Effectiveness and Cytotoxicity of the Antibiotic-Loaded Chitosan: ECM Scaffolds

Goller, Shayla and Turner, Neill J. (2020) The Antimicrobial Effectiveness and Cytotoxicity of the Antibiotic-Loaded Chitosan: ECM Scaffolds. Applied Sciences, 10 (10). p. 3446. ISSN 2076-3417

Published Version

Download (2MB) | Preview


<b>Background:</b> The development of multifunctional wound dressings with the ability to control hemostasis, limit infection and promote rapid wound healing and constructive tissue remodeling has been a challenge for many years. In view of these challenges, a hybrid scaffold platform was developed that combined two different extracellular matrices (ECM): ECM from decellularized mammalian tissue and ECM (chitosan) from crustaceans. Both types of ECM have well established clinical benefits that support and promote wound healing and control hemostasis. This scaffold platform could also be augmented with antibiotics to provide bactericidal activity directly to the wound site. <b>Methods:</b> Four different scaffold formulations were developed containing chitosan supplemented with either 20% or 50% urinary bladder matrix (UBM) hydrogel or 1% (<i>w</i>/<i>v</i>) or 10% (<i>w</i>/<i>v</i>) UBM–ECM particulates. 100% chitosan scaffolds were used as controls. The scaffolds were augmented with either minocycline or rifampicin. <i>E</i><i>scherichia</i><i> Coli</i> and <i>Staphylococcus</i> <i>Aureus </i>were used to assesses antimicrobial efficacy and duration of activity, while neutral red uptake assays were performed to establish direct and indirect cytotoxicity. <b>Results: </b>Results showed that scaffold handling properties, scaffold integrity over time and the efficacy and release rate of loaded antibiotics could be modified by altering scaffold composition. Moreover, antibiotics were easily released from the scaffold and could remain effective for up to 24 h by modifying the scaffold composition. Variable results with cytotoxicity testing show that further work is required to optimize the scaffold formulations but these proof of principle experiments suggest that these scaffolds have potential as bioactive wound dressings.


Social Networking:
Share |


Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Turner, Neill
Centers: Other Centers, Institutes, Offices, or Units > McGowan Institute for Regenerative Medicine
Date: 16 May 2020
Date Type: Publication
Journal or Publication Title: Applied Sciences
Volume: 10
Number: 10
Publisher: MDPI AG
Page Range: p. 3446
DOI or Unique Handle: 10.3390/app10103446
Schools and Programs: School of Medicine > Surgery
Refereed: Yes
Uncontrolled Keywords: chitosan, extracellular matrix, antimicrobial, biocompatible, scaffold, wound healing
ISSN: 2076-3417
Official URL:
Article Type: Research Article
Date Deposited: 19 May 2021 18:19
Last Modified: 19 May 2021 18:19


Monthly Views for the past 3 years

Plum Analytics

Actions (login required)

View Item View Item