O'Donnell, Benjamen and Al-Ghadban, Sara and Ives, Clara and L'Ecuyer, Michael and Monjure, Tia and Romero-Lopez, Monica and Li, Zhong and Goodman, Stuart and Lin, Hang and Tuan, Rocky and Bunnell, Bruce
(2020)
Adipose Tissue-Derived Stem Cells Retain Their Adipocyte Differentiation Potential in Three-Dimensional Hydrogels and Bioreactors.
Biomolecules, 10 (7).
p. 1070.
ISSN 2218-273X
Abstract
Osteoarthritis (OA) is a common joint disorder with a significant economic and healthcare impact. The knee joint is composed of cartilage and the adjoining bone, a synovial capsule, the infrapatellar fat pad (IPFP), and other connective tissues such as tendons and ligaments. Adipose tissue has recently been highlighted as a major contributor to OA through strong inflammation mediating effects. In this study, methacrylated gelatin (GelMA) constructs seeded with adipose tissue-derived mesenchymal stem cells (ASCs) and cultured in a 3D printed bioreactor were investigated for use in microphysiological systems to model adipose tissue in the knee joint. Four patient-derived ASC populations were seeded at a density of 20 million cells/mL in GelMA. Live/Dead and boron-dipyrromethene/4′,6-diamidino-2-phenylindole (BODIPY/DAPI) staining of cells within the constructs demonstrated robust cell viability after 28 days in a growth (control) medium, and robust cell viability and lipid accumulation in adipogenic differentiation medium. qPCR gene expression analysis and protein analysis demonstrated an upregulated expression of key adipogenesis-associated genes. Overall, these data indicate that ASCs retain their adipogenic potential when seeded within GelMA hydrogels and cultured within perfusion bioreactors, and thus can be used in a 3D organ-on-a-chip system to study the role of the IPFP in the pathobiology of the knee OA.
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Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID |
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O'Donnell, Benjamen | | | | Al-Ghadban, Sara | | | | Ives, Clara | | | | L'Ecuyer, Michael | | | | Monjure, Tia | | | | Romero-Lopez, Monica | | | | Li, Zhong | alanzhongli@pitt.edu | alanzhongli | | Goodman, Stuart | | | | Lin, Hang | hal46@pitt.edu | hal46 | | Tuan, Rocky | tuanr@cuhk.edu.hk | | | Bunnell, Bruce | | | |
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Date: |
1 July 2020 |
Date Type: |
Publication |
Journal or Publication Title: |
Biomolecules |
Volume: |
10 |
Number: |
7 |
Publisher: |
MDPI AG |
Page Range: |
p. 1070 |
DOI or Unique Handle: |
10.3390/biom10071070 |
Schools and Programs: |
School of Medicine > Orthopaedic Surgery |
Refereed: |
Yes |
Uncontrolled Keywords: |
microphysiological system, tissue-on-a-chip, adipose stem cells, adipocytes, methacrylated gelatin, osteoarthritis |
ISSN: |
2218-273X |
Official URL: |
http://dx.doi.org/10.3390/biom10071070 |
Funders: |
National Institutes of Health, National Science Foundation |
Article Type: |
Research Article |
Date Deposited: |
26 May 2021 16:32 |
Last Modified: |
26 May 2021 16:32 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/41165 |
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