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Adipose Tissue-Derived Stem Cells Retain Their Adipocyte Differentiation Potential in Three-Dimensional Hydrogels and Bioreactors

O'Donnell, Benjamen and Al-Ghadban, Sara and Ives, Clara and L'Ecuyer, Michael and Monjure, Tia and Romero-Lopez, Monica and Li, Zhong and Goodman, Stuart and Lin, Hang and Tuan, Rocky and Bunnell, Bruce (2020) Adipose Tissue-Derived Stem Cells Retain Their Adipocyte Differentiation Potential in Three-Dimensional Hydrogels and Bioreactors. Biomolecules, 10 (7). p. 1070. ISSN 2218-273X

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Osteoarthritis (OA) is a common joint disorder with a significant economic and healthcare impact. The knee joint is composed of cartilage and the adjoining bone, a synovial capsule, the infrapatellar fat pad (IPFP), and other connective tissues such as tendons and ligaments. Adipose tissue has recently been highlighted as a major contributor to OA through strong inflammation mediating effects. In this study, methacrylated gelatin (GelMA) constructs seeded with adipose tissue-derived mesenchymal stem cells (ASCs) and cultured in a 3D printed bioreactor were investigated for use in microphysiological systems to model adipose tissue in the knee joint. Four patient-derived ASC populations were seeded at a density of 20 million cells/mL in GelMA. Live/Dead and boron-dipyrromethene/4′,6-diamidino-2-phenylindole (BODIPY/DAPI) staining of cells within the constructs demonstrated robust cell viability after 28 days in a growth (control) medium, and robust cell viability and lipid accumulation in adipogenic differentiation medium. qPCR gene expression analysis and protein analysis demonstrated an upregulated expression of key adipogenesis-associated genes. Overall, these data indicate that ASCs retain their adipogenic potential when seeded within GelMA hydrogels and cultured within perfusion bioreactors, and thus can be used in a 3D organ-on-a-chip system to study the role of the IPFP in the pathobiology of the knee OA.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
O'Donnell, Benjamen
Al-Ghadban, Sara
Ives, Clara
L'Ecuyer, Michael
Monjure, Tia
Romero-Lopez, Monica
Li, Zhongalanzhongli@pitt.edualanzhongli
Goodman, Stuart
Lin, Hanghal46@pitt.eduhal46
Bunnell, Bruce
Date: 1 July 2020
Date Type: Publication
Journal or Publication Title: Biomolecules
Volume: 10
Number: 7
Publisher: MDPI AG
Page Range: p. 1070
DOI or Unique Handle: 10.3390/biom10071070
Schools and Programs: School of Medicine > Orthopaedic Surgery
Refereed: Yes
Uncontrolled Keywords: microphysiological system, tissue-on-a-chip, adipose stem cells, adipocytes, methacrylated gelatin, osteoarthritis
ISSN: 2218-273X
Official URL:
Funders: National Institutes of Health, National Science Foundation
Article Type: Research Article
Date Deposited: 26 May 2021 16:32
Last Modified: 26 May 2021 16:32


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