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Detection of Cardiac Biomarkers Using Single Polyaniline Nanowire-Based Conductometric Biosensors

Lee, Innam and Luo, Xiliang and Huang, Jiyong and Cui, Xinyan Tracy and Yun, Minhee (2012) Detection of Cardiac Biomarkers Using Single Polyaniline Nanowire-Based Conductometric Biosensors. Biosensors, 2 (2). pp. 205-220. ISSN 2079-6374

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The detection of myoglobin (Myo), cardiac troponin I (cTnI), creatine kinase-MB (CK-MB), and b-type natriuretic peptide (BNP) plays a vital role in diagnosing cardiovascular diseases. Here we present single site-specific polyaniline (PANI) nanowire biosensors that can detect cardiac biomarkers such as Myo, cTnI, CK-MB, and BNP with ultra-high sensitivity and good specificity. Using single PANI nanowire-based biosensors integrated with microfluidic channels, very low concentrations of Myo (100 pg/mL), cTnI (250 fg/mL), CK-MB (150 fg/mL), and BNP (50 fg/mL) were detected. The single PANI nanowire-based biosensors displayed linear sensing profiles for concentrations ranging from hundreds (fg/mL) to tens (ng/mL). In addition, devices showed a fast (few minutes) response satisfying respective reference conditions for Myo, cTnI, CK-MB, and BNP diagnosis of heart failure and for determining the stage of the disease. This single PANI nanowire-based biosensor demonstrated superior biosensing reliability with the feasibility of label free detection and improved processing cost efficiency due to good biocompatibility of PANI to monoclonal antibodies (mAbs). Therefore, this development of single PANI nanowire-based biosensors can be applied to other biosensors for cancer or other diseases.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Lee, Innam
Luo, Xiliang
Huang, Jiyong
Cui, Xinyan Tracyxic11@pitt.eduxic11
Yun, Minheemiy16@pitt.edumiy16
Date: 14 May 2012
Date Type: Publication
Journal or Publication Title: Biosensors
Volume: 2
Number: 2
Publisher: MDPI AG
Page Range: pp. 205-220
DOI or Unique Handle: 10.3390/bios2020205
Schools and Programs: Swanson School of Engineering > Electrical and Computer Engineering
Refereed: Yes
Uncontrolled Keywords: myoglobin, cardiac troponin I, creatine kinase-MB, b-type natriuretic peptide, polyaniline, nanowire, conductometric biosensing
ISSN: 2079-6374
Official URL:
Article Type: Research Article
Date Deposited: 28 May 2021 14:18
Last Modified: 28 May 2021 14:18


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