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Paternal Preconception Every-Other-Day Ethanol Drinking Alters Behavior and Ethanol Consumption in Offspring

Beeler, Erik and Nobile, Zachary and Homanics, Gregg (2019) Paternal Preconception Every-Other-Day Ethanol Drinking Alters Behavior and Ethanol Consumption in Offspring. Brain Sciences, 9 (3). p. 56. ISSN 2076-3425

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Alcohol use disorder is a devastating disease with a complex etiology. Recent preclinical studies have revealed that paternal preconception chronic intermittent ethanol (EtOH) exposure via vaporized EtOH altered drinking behaviors and sensitivity to EtOH selectively in male offspring. In the current study, we used a voluntary oral route of paternal preconception EtOH exposure, i.e., intermittent every-other-day two-bottle choice drinking, and tested offspring for behavioral alterations. Fifteen EtOH drinking sires and 10 control sires were mated to EtOH naïve females to produce EtOH-sired and control-sired offspring. These offspring were tested using the elevated plus maze, open field, drinking in the dark, and unlimited access two-bottle choice assays. We found that paternal preconception every-other-day two-bottle choice drinking resulted in reduced EtOH consumption selectively in male offspring in the drinking in the dark assay compared to control-sired offspring. No differences were detected in either sex in the unlimited access two-bottle choice and elevated plus maze assays. Open field analysis revealed complex changes in basal behavior and EtOH-induced behaviors that were sex specific. We concluded that paternal preconception voluntary EtOH consumption has persistent effects that impact the next generation. This study adds to a growing appreciation that one’s behavioral response to EtOH and EtOH drinking behavior are impacted by EtOH exposure of the prior generation.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Beeler, Erik
Nobile, Zachary
Centers: Other Centers, Institutes, Offices, or Units > Center for Neuroscience
Date: 6 March 2019
Date Type: Publication
Journal or Publication Title: Brain Sciences
Volume: 9
Number: 3
Publisher: MDPI AG
Page Range: p. 56
DOI or Unique Handle: 10.3390/brainsci9030056
Schools and Programs: School of Medicine > Anesthesiology
Refereed: Yes
Uncontrolled Keywords: alcohol; EtOH; alcohol use disorder; alcoholism; epigenetics; epigenetic inheritance; intergenerational effect
ISSN: 2076-3425
Official URL:
Funders: National Institutes of Health
Article Type: Research Article
Date Deposited: 28 May 2021 14:18
Last Modified: 28 May 2021 14:18


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