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Isolation and primary cultures of human intrahepatic bile ductular epithelium

Demetris, AJ and Markus, BH and Saidman, S and Fung, JJ and Makowka, L and Graner, S and Duquesnoy, R and Starzl, TE (1988) Isolation and primary cultures of human intrahepatic bile ductular epithelium. In Vitro Cellular & Developmental Biology, 24 (5). 464 - 470. ISSN 0883-8364

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Abstract

A technique for the isolation of human intrahepatic bile ductular epithelium, and the establishment of primary cultures using a serum- and growth-factor-supplemented medium combined with a connective tissue substrata is described. Initial cell isolates and monolayer cultures display phenotypic characteristics of biliary epithelial cells (low molecular weight prekeratin positive; albumin, alphafetoprotein, and Factor VIII-related antigen negative). Ultrastructural features of the cultured cells show cell polarization with surface microvilli, numerous interepithelial junctional complexes and cytoplasmic intermediate prekeratin filaments. © 1988 Tissue Culture Association, Inc.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Demetris, AJ
Markus, BH
Saidman, S
Fung, JJ
Makowka, L
Graner, S
Duquesnoy, R
Starzl, TEtes11@pitt.eduTES11
Centers: Other Centers, Institutes, or Units > Thomas E. Starzl Transplantation Institute
Date: 1 May 1988
Date Type: Publication
Journal or Publication Title: In Vitro Cellular & Developmental Biology
Volume: 24
Number: 5
Page Range: 464 - 470
DOI or Unique Handle: 10.1007/bf02628499
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0883-8364
Other ID: uls-drl:31735062128685, Starzl CV No. 816
Date Deposited: 08 Apr 2010 17:13
Last Modified: 13 Oct 2017 21:57
URI: http://d-scholarship.pitt.edu/id/eprint/4202

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