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Recruitment of reovirus RNAs to viral factories

Lee, Christopher (2022) Recruitment of reovirus RNAs to viral factories. Doctoral Dissertation, University of Pittsburgh. (Unpublished)

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Viruses face monumental challenges when colonizing a cell. They are much smaller
than the cells they infect, yet they can efficiently replicate in relatively-large complex
environments. Viruses thrive in such environments by constructing structures optimized
for producing more viruses. These intracellular structures, called viral factories,
concentrate viral and host factors that enable efficient viral packaging and replication.
The mammalian orthoreovirus (reovirus) is a double stranded RNA virus that builds
distinct cytoplasmic factories in infected cells. However, mechanisms underlying the
formation of these structures are not well defined. Two reovirus nonstructural proteins,
σNS and µNS, are required for viral factory formation, but the function of σNS in this
process has been enigmatic. This thesis is focuses on defining the role of σNS in viral
factory formation. I determined that σNS is required to recruit viral mRNAs into factory
scaffolds. Additionally, I describe how σNS forms a helical oligomer. Lastly, I discovered
that σNS is not solely responsible for regulating RNAs recruited to factories. My findings
define a function for σNS in factory formation and provide the foundation for additional
studies that will enhance our understanding of reovirus factory formation and replication


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Lee, Christopherchl246@pitt.educhl246
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairKinchington, Paul
Committee MemberAtianand, Maninjay
Committee MemberBrodsky, Jeff
Committee MemberLakdawala, Seema
Date: 18 January 2022
Date Type: Publication
Defense Date: 28 September 2021
Approval Date: 18 January 2022
Submission Date: 12 December 2021
Access Restriction: 2 year -- Restrict access to University of Pittsburgh for a period of 2 years.
Number of Pages: 154
Institution: University of Pittsburgh
Schools and Programs: School of Medicine > Microbiology and Immunology
Degree: PhD - Doctor of Philosophy
Thesis Type: Doctoral Dissertation
Refereed: Yes
Uncontrolled Keywords: Reovirus, factories, RNAs, phase-separation
Date Deposited: 18 Jan 2022 16:31
Last Modified: 18 Jan 2022 16:31

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