Lee, Christopher
(2022)
Recruitment of reovirus RNAs to viral factories.
Doctoral Dissertation, University of Pittsburgh.
(Unpublished)
This is the latest version of this item.
Abstract
Viruses face monumental challenges when colonizing a cell. They are much smaller
than the cells they infect, yet they can efficiently replicate in relatively-large complex
environments. Viruses thrive in such environments by constructing structures optimized
for producing more viruses. These intracellular structures, called viral factories,
concentrate viral and host factors that enable efficient viral packaging and replication.
The mammalian orthoreovirus (reovirus) is a double stranded RNA virus that builds
distinct cytoplasmic factories in infected cells. However, mechanisms underlying the
formation of these structures are not well defined. Two reovirus nonstructural proteins,
σNS and µNS, are required for viral factory formation, but the function of σNS in this
process has been enigmatic. This thesis is focuses on defining the role of σNS in viral
factory formation. I determined that σNS is required to recruit viral mRNAs into factory
scaffolds. Additionally, I describe how σNS forms a helical oligomer. Lastly, I discovered
that σNS is not solely responsible for regulating RNAs recruited to factories. My findings
define a function for σNS in factory formation and provide the foundation for additional
studies that will enhance our understanding of reovirus factory formation and replication
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Details
Item Type: |
University of Pittsburgh ETD
|
Status: |
Unpublished |
Creators/Authors: |
|
ETD Committee: |
Title | Member | Email Address | Pitt Username | ORCID |
---|
Committee Chair | Kinchington, Paul | | | | Committee Member | Atianand, Maninjay | | | | Committee Member | Brodsky, Jeff | | | | Committee Member | Lakdawala, Seema | | | |
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Date: |
18 January 2022 |
Date Type: |
Publication |
Defense Date: |
28 September 2021 |
Approval Date: |
18 January 2022 |
Submission Date: |
12 December 2021 |
Access Restriction: |
2 year -- Restrict access to University of Pittsburgh for a period of 2 years. |
Number of Pages: |
154 |
Institution: |
University of Pittsburgh |
Schools and Programs: |
School of Medicine > Microbiology and Immunology |
Degree: |
PhD - Doctor of Philosophy |
Thesis Type: |
Doctoral Dissertation |
Refereed: |
Yes |
Uncontrolled Keywords: |
Reovirus, factories, RNAs, phase-separation |
Date Deposited: |
18 Jan 2022 16:31 |
Last Modified: |
18 Jan 2024 06:15 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/42168 |
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