Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form
D-Scholarship @ Pitt Banner and Links to Homepage

Molecular Characterization of Human Spermatogonial Stem Cells

Munyoki, Sarah (2022) Molecular Characterization of Human Spermatogonial Stem Cells. Doctoral Dissertation, University of Pittsburgh. (Unpublished)

[img] PDF
Restricted to University of Pittsburgh users only until 1 July 2024.
Download (4MB) | Request a Copy

Abstract

Mammalian spermatogenesis is a complex, highly productive process generating millions of sperm per day. Spermatogonial stem cells (SSCs) are at the foundation of spermatogenesis and can either self-renew, producing more SSCs, or differentiate to eventually produce sperm. This unique ability of SSCs renders them a promising tool in developing therapies for the treatment of male infertility. However, difficulties in unequivocally identifying human SSCs (hSSCs) and complexity in replicating their functions in vitro have hampered our understanding of hSSCs and our ability to utilize them for assisted reproductive technologies. This study employed single-cell RNA sequencing (scRNA-seq), immunohistochemistry, flow cytometry, xenotransplantation, and cell culture techniques to molecularly characterize putative human hSSCs. Identifying the protein markers of hSSCs and the signaling pathways regulating their self-renewal and proliferation will facilitate the development of efficient, effective, and safe SSC-based therapies to treat male infertility.

Share

Citation/Export:
Social Networking:
Share |

Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Munyoki, Sarahskm53@pitt.eduskm530000-0001-6608-9130
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairChu, Tianjiaotchu@mwri.magee.edu
Thesis AdvisorOrwig, Kylekorwig@mwri.magee.edukeo6
Committee MemberYanowitz, Judyyanowitzjl@mwri.magee.edujly23
Committee MemberCarvunis, Anne-Ruxandraanc201@pitt.eduanc201
Committee MemberNeil, Hukriedehukriede@pitt.eduhukriede
Date: 1 July 2022
Date Type: Publication
Defense Date: 13 October 2021
Approval Date: 1 July 2022
Submission Date: 26 April 2022
Access Restriction: 2 year -- Restrict access to University of Pittsburgh for a period of 2 years.
Number of Pages: 134
Institution: University of Pittsburgh
Schools and Programs: School of Medicine > Integrative Systems Biology
Degree: PhD - Doctor of Philosophy
Thesis Type: Doctoral Dissertation
Refereed: Yes
Uncontrolled Keywords: Spermatogonial Stem cells, Infertility
Date Deposited: 01 Jul 2022 14:02
Last Modified: 01 Jul 2022 14:02
URI: http://d-scholarship.pitt.edu/id/eprint/42761

Metrics

Monthly Views for the past 3 years

Plum Analytics

Actions (login required)

View Item View Item