Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

A Study on Temporomandibular Joint Tissue Characterization, Degeneration and Regeneration

Li, Wuyang (2022) A Study on Temporomandibular Joint Tissue Characterization, Degeneration and Regeneration. Doctoral Dissertation, University of Pittsburgh. (Unpublished)

[img] PDF
Restricted to University of Pittsburgh users only until 9 August 2024.

Download (3MB) | Request a Copy


This dissertation investigated three different topics, each with a separate aim, in the field
of temporomandibular joint (TMJ) research, from characterization (Aim 1), to degeneration (Aim
2), to regeneration (Aim 3).
In Aim 1, I explored the validity and trueness of fit for using the transverse isotropic
biphasic (TIB) and Kelvin models for characterization of the tensile properties of the TMJ discs
from pig and goat. Incremental stress-relaxation test was performed on pig TMJ discs and a singlestep stress-relaxation test was performed pig and goat TMJ discs. The TIB model yielded reliable
fits only at low strain, while the Kelvin models yielded good fits at both low and high strain, with
the 2nd order generalized Kelvin model yielding the best fit. When comparing pig to goat TMJ
discs in 10% strain stress-relaxation test, the TIB model did not fit well for this larger step. This
result suggested that TIB might not be a good candidate for characterizing the tensile properties of
the TMJ discs, and other biphasic models can be tested in the future.
In Aim 2, I tried to identify the best bite-changing approach for establishing degenerative
joint disease (DJD) in rats. Three different bite-raising approaches were applied to rats for 28 days.
TMJ condyle gross appearance and histology was assessed. The results suggest that all splinting
methods can induce DJD, but resin molar splint was associated with the most consistent and severe
changes as observed with side-to-side difference, and thus is recommended for future rat DJD
In Aim 3, I focused on TMJ regeneration, aiming to gain further knowledge in cell type
and scaffold material interactions in chondrogenesis. Two cell populations were harvested from
the mandibular condyle fibrocartilage and seeded into two different scaffolds for 3 weeks. Gross
appearance, histology, biochemistry, and mechanical tests were performed at different time points.
Results showed that gelatin (GEL) scaffolds induced chondrogenesis, but a gelatin blend with
other polymers (PGH) did not. No major significant difference was observed between cell types,
indicating that PGH and GEL play a more important role in the process of chondrogenesis than
the tested cell populations.


Social Networking:
Share |


Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Li, Wuyangwul4@pitt.eduwul4
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairAlmarza, Alejandroaja19@pitt.eduaja19
Taboas, Juanjmt106@pitt.edujmt106
Szabo-Rogers, Heatherhsrogers@pitt.eduhsrogers
Gold, Michaelmsg22@pitt.edumsg22
Date: 9 August 2022
Date Type: Publication
Defense Date: 27 May 2022
Approval Date: 9 August 2022
Submission Date: 13 June 2022
Access Restriction: 2 year -- Restrict access to University of Pittsburgh for a period of 2 years.
Number of Pages: 7
Institution: University of Pittsburgh
Schools and Programs: School of Dental Medicine > Dental Science
Degree: PhD - Doctor of Philosophy
Thesis Type: Doctoral Dissertation
Refereed: Yes
Uncontrolled Keywords: Temporomandibular joint disorder; bite-changing splint; Degenerative joint disease; tensile test; TMJ disc tensile models; mandibular condyle cartilage regeneration; hydrogel
Related URLs:
Date Deposited: 09 Aug 2022 18:53
Last Modified: 09 Aug 2022 18:53


Monthly Views for the past 3 years

Plum Analytics

Actions (login required)

View Item View Item