Dioguardi, Jasmine, S
(2022)
Detecting Remission in Ulcerative Colitis Patients: Finding A Universal Definition.
Master's Thesis, University of Pittsburgh.
(Unpublished)
Abstract
Ulcerative colitis (UC) is a form of inflammatory bowel disease with an unknown etiology and is characterized by chronic inflammation and ulcer formation localized in the mucosal lining of the large intestine and rectum. More than 2 million individuals in North America are currently affected, and, with the incidence rate steadily increasing worldwide, the demand for improvements in detection and therapeutics is of public health importance. One major obstacle to UC treatment is the lack of a uniform definition for remission, as classification is typically dependent on its purpose. My work aims to identify changes in the epigenome and transcriptome in various patient sample stratifications to determine a uniform remission definition in a clinical setting. Intestinal epithelial cells were collected from patient biopsies in a histologically defined active or remissive disease state, with non-UC patient biopsies used as a control. To identify differences in select histone posttranslational modifications and transcription factor binding profiles on chromatin, samples were processed using CUT&RUN. In parallel, to determine changes in gene expression, RNA-seq was performed on the same patient samples. We expect that identifying changes in the epigenome and subsequent gene expression is a substantial method for classifying patients in remission.
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| Item Type: |
University of Pittsburgh ETD
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| Status: |
Unpublished |
| Creators/Authors: |
|
| ETD Committee: |
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| Date: |
1 July 2022 |
| Date Type: |
Publication |
| Defense Date: |
28 June 2022 |
| Approval Date: |
1 July 2022 |
| Submission Date: |
28 June 2022 |
| Access Restriction: |
No restriction; Release the ETD for access worldwide immediately. |
| Number of Pages: |
83 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
School of Public Health > Human Genetics |
| Degree: |
MS - Master of Science |
| Thesis Type: |
Master's Thesis |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
CUT&RUN, RNA-seq, ulcerative colitis, IBD |
| Date Deposited: |
01 Jul 2022 18:41 |
| Last Modified: |
01 Jul 2022 18:41 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/43184 |
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