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Beyond Histones: Discovery of Lysine-Mediated Protein-Protein Interactions via Site Specific Photochemistry

Wagner, Shana (2022) Beyond Histones: Discovery of Lysine-Mediated Protein-Protein Interactions via Site Specific Photochemistry. Doctoral Dissertation, University of Pittsburgh. (Unpublished)

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Abstract

Epigenetic readers, writers, and erasers are responsible for the dynamic state of the genome, allowing vital cellular processes to take place. Although the role of modified lysines in histones is widely understood, the same cannot be said for non-histones. The following research discusses the use of photoactive readers and erasers to capture novel interacting partners of BPTF (Bromodomain PHD Finger Transcription Factor), BET (Bromodomain and extraterminal domain) bromodomains, and KDM4s (lysine demethylase 4s), as well as the subsequent biochemical and cell-based experiments used to confirm and further characterize these interactions. The vast network of epigenetic proteins is not yet understood, but with further techniques and studies the scientific community gets closer to fully understanding the cell.

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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Wagner, Shanasbw29@pitt.edusbw29
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairIslam, Kabirulkai27@pitt.edukai27
Committee MemberChilders, Sethwschild@pitt.eduwschild
Committee MemberWeber, Stephensweber@pitt.edusweber
Committee MemberGoetzman, Ericeric.goetzman@chp.edueric.goetzman
Date: 12 October 2022
Date Type: Publication
Defense Date: 20 July 2022
Approval Date: 12 October 2022
Submission Date: 27 July 2022
Access Restriction: 2 year -- Restrict access to University of Pittsburgh for a period of 2 years.
Number of Pages: 166
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Degree: PhD - Doctor of Philosophy
Thesis Type: Doctoral Dissertation
Refereed: Yes
Uncontrolled Keywords: epigenetics, BRD, KDM4, methylation, demethylation, acetylation, deacetylation
Date Deposited: 12 Oct 2022 20:26
Last Modified: 12 Oct 2022 20:26
URI: http://d-scholarship.pitt.edu/id/eprint/43397

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