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Response of Cultured Hepatocytes to a Hepatomitogen after Initiation by Conditioned Medium or Other Factors

Ove, P and Francavilla, A and Coetzee, ML and Makowka, L and Starzl, TE (1989) Response of Cultured Hepatocytes to a Hepatomitogen after Initiation by Conditioned Medium or Other Factors. Cancer Research, 49 (1). 98 - 103. ISSN 0008-5472

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Abstract

Injection of a substantially purified hepatomitogen into recipient rats that had 40% of their liver removed resulted in a significant stimulation of hepatic DNA synthesis as determined by the labeling index and the mitotic index. Normal or sham-operated rats did not respond to the injection of the mitogen. The extraction and partial purification of this hepatomitogen have previously been reported (A. Francavilla et al., Cancer Res., 47:5600-5605,1987). Addition of the factor to an epithelial-like liver-derived cell line in culture (clone 9) or to a hepatoma cell line (HTC-SR) resulted in a dose-dependent stimulation of DNA synthesis. Hepatocytes in primary culture, on the other hand, were not stimulated by the addition of the factor. However, when the mitogen was added to hepatocytes in primary culture, together with conditioned medium, obtained from the responsive cell lines, a significant stimulation of DNA synthesis could be demonstrated in hepatocytes in culture. The stimulation was dose dependent with respect to the mitogen, was abolished by 10 mm hydroxyurea, and was independent of epidermal growth factor. The conditioned medium could be replaced by a protein factor extracted from the two cell lines as previously reported (P. Ove et al., J. Cell. Physiol., 131:165-174,1987). It appears that a cofactor is provided by the conditioned medium or by the cell extract, enabling the hepatomitogen to act on hepatocytes in primary culture. © 1989, American Association for Cancer Research. All rights reserved.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Ove, P
Francavilla, A
Coetzee, ML
Makowka, L
Starzl, TEtes11@pitt.eduTES11
Centers: Other Centers, Institutes, or Units > Thomas E. Starzl Transplantation Institute
Date: 1 January 1989
Date Type: Publication
Journal or Publication Title: Cancer Research
Volume: 49
Number: 1
Page Range: 98 - 103
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0008-5472
Other ID: uls-drl:31735062131028, Starzl CV No. 954
Date Deposited: 08 Apr 2010 17:16
Last Modified: 05 Dec 2017 17:00
URI: http://d-scholarship.pitt.edu/id/eprint/4340

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