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Development of Two-Dimensional Photonic Crystal Hydrogel Sensors for Biomolecular Detection

Jang, Kyeongwoo (2023) Development of Two-Dimensional Photonic Crystal Hydrogel Sensors for Biomolecular Detection. Doctoral Dissertation, University of Pittsburgh. (Unpublished)

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The work reported in this dissertation discusses the recent advancements in the development of 2DPC hydrogel sensors for biomolecular detection. The 2DPC hydrogel sensors are fabricated by incorporating 2DPC into responsive hydrogels. Each hydrogel sensor is functionalized with specific recognition groups that interact with the desired target, causing the hydrogel to undergo volume phase transitions (VPTs). We measure the target-induced hydrogel VPTs by monitoring the 2DPC light diffraction and report the analyte concentrations. This sensing platform is simple, does not require sophisticated instrumentation, and could be used in resource-limited environments and point-of-care testing.
We demonstrated the use of oxyamine recognition groups for the detection of phenylpyruvate, an enzymatic reaction product between phenylalanine and phenylalanine dehydrogenase. The chemoselective oxime reaction between the hydrogel oxyamines and phenylpyruvate covalently modifies the hydrogel structure and induces the hydrogel VPTs. This 2DPC hydrogel sensor could be further used to develop a phenylalanine sensor for patients with phenylketonuria and to develop a lactate sensor to evaluate the development of sepsis.
We also developed two other mechanisms for small molecule detection utilizing DNA aptamers as molecular recognition groups. Upon binding targets, the DNA aptamers that are attached to the hydrogel network undergo conformational changes, triggering the hydrogel VPTs. We hypothesize that this sensing motif is generalizable and that other sensors can be easily fabricated by simply exchanging the aptamer recognition group. We plan to continue to develop this sensing platform for the detection of other small molecules and proteins of interest.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Jang, Kyeongwookyj9@pitt.edukyj9
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairAsher,
Committee MemberHorne,
Committee MemberLaaser,
Committee MemberVelankar,
Date: 25 January 2023
Date Type: Publication
Defense Date: 28 September 2022
Approval Date: 25 January 2023
Submission Date: 30 September 2022
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Number of Pages: 161
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Degree: PhD - Doctor of Philosophy
Thesis Type: Doctoral Dissertation
Refereed: Yes
Uncontrolled Keywords: photonic crystal, hydrogel, responsive polymer, molecular recognition group, phenylpyruvate, aptamer, adenosine
Date Deposited: 25 Jan 2023 20:12
Last Modified: 25 Jan 2023 20:12


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