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Strategies to Treat Periodontal Tissues and Regenerate Pulp-Dentin Complex

Khayat, Arwa (2023) Strategies to Treat Periodontal Tissues and Regenerate Pulp-Dentin Complex. Doctoral Dissertation, University of Pittsburgh. (Unpublished)

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Periodontal disease (PD) and pulp infections are among the most common oral diseases affecting adults in the United States. PD is considered as one of the leading causes of tooth and alveolar bone loss and has been implicated in a number of systemic conditions.
It is well established that local host response
is responsible for PD progression and recurrence. Thus, developing a therapy to modulate the local host response has become a new exciting target for treating PD. Recently, Mg has been regarded as an important anti-inflammatory and immuno-modulatory cation. In this proposal we evaluate in vitro and in vivo the role of Mg as an anti-inflammatory to treat PD and to promote calcification as a direct pulp capping agent. For the in vitro experiments, gingival fibroblast cells were used. The cells were incubated in the presence of lipopolysaccharide (LPS) to induce an inflammatory response. In the treatment group Mg was added to the cells with or without Mg and was shown to reduce the inflammatory response as evidenced by a statistical significant decrease in expression of IL-1 and IL6. We then assessed if Mg release from degradable microparticles (MPs) in BLAB/C mouse ligature induced periodontitis will prevent the disease progression. When the MPs were injected at the same time when the ligatures were placed (preventive model) the disease progression was assessed using micro computed tomography (CT) and the data showed significantly less bone loss in the treated group compared to the untreated control groups. The effect of Mg+2
on pre-odontoblast cells was assessed in vitro for its osteogenic properties. The
osteogenic/odontogenic markers did not show any difference between all study groups at day three except for bglap, which showed a statistically significant higher gene expression compared to the control. In summary, the data showed that the delivery of Mg could be a viable potential treatment option for
periodontal disease as well as a pulp capping technology.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Khayat, Arwaark151@pitt.eduark151
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairBeniash, Eliaebeniash@pitt.eduebeniash
Thesis AdvisorSfeir, Charlescsfeir@pitt.educsfeir
Committee MemberAlmarza, Alejandroaja19@pitt.eduaja19
Committee MemberRay, Herberthlr21@pitt.eduhlr21
Date: 31 January 2023
Date Type: Publication
Defense Date: 22 November 2022
Approval Date: 31 January 2023
Submission Date: 12 December 2022
Access Restriction: 2 year -- Restrict access to University of Pittsburgh for a period of 2 years.
Number of Pages: 126
Institution: University of Pittsburgh
Schools and Programs: School of Dental Medicine > Dental Science
Degree: PhD - Doctor of Philosophy
Thesis Type: Doctoral Dissertation
Refereed: Yes
Uncontrolled Keywords: periodontitis, inflammation, dentin-pulp complex
Date Deposited: 31 Jan 2023 17:48
Last Modified: 31 Jan 2023 17:48


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