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Modificatoin of a Commercially Available Dot Blot Hybridization Assay for the Detection of Human Papillomarvirus

Ondiek, Sally J. (1992) Modificatoin of a Commercially Available Dot Blot Hybridization Assay for the Detection of Human Papillomarvirus. Master's Thesis, University of Pittsburgh.

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Over the past decade there has been increasing evidence indicating that certain Human Papillomaviruses {HPV) are important etiological agents involved in the development of cancer of the cervix {12,13,36). HPV types 6 and 11 tend to be associated with benign genital warts and/or low-grade cervical intraepithelial neoplasia {CIN); whereas, HPV types 16, 18, 31,33 and 35 are more prevalent in high grade CIN {53,63,65). Based upon this association, it has been hypothesized that the presence of certain HPV genotypes may help predict the progression from various stages of CIN to invasive cervical cancer.

Detection of Papillomavirus infections present difficult diagnostic problems. HPV cannot be cultivated in vitro and viral antigens are only rarely present in high grade CIN lesions. HPV infection is suspected by the presence of typical morphological changes in the epithelial cells detectable by cytology or histopathology. Specific identification of HPV infection is possible by nucleic acid hybridization with type specific probes (55). Several DNA hybridization methods are available, including Southern blotting {SB), dot blotting (DB), filter in-situ hybridization (FISH) and in-situ hybridization {ISH) on tissue sections {58).

The purpose of the present study was to evaluate the effect of three modifications made to the Virapap HPV DNA filter hybridization assay (Digene Diagnostics; Gaithersburg, MD) in detecting HPV DNA from cervical scrape specimens. The three modifications included increasing the amount of specimen tested, increasing the hybridization time and increasing the exposure time.


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Item Type: Other Thesis, Dissertation, or Long Paper (Master's Thesis)
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Ondiek, Sally J.
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairSzuminsky, Neil
Committee MemberMatuscak, Rosemarie
Committee MemberMeyer, Mike
Committee MemberAmortegui, Antonio
Date: 15 April 1992
Date Type: Completion
Defense Date: 15 April 1992
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Number of Pages: 57
Institution: University of Pittsburgh
Schools and Programs: School of Health and Rehabilitation Sciences > Rehabilitation Science
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Date Deposited: 13 Dec 2022 20:53
Last Modified: 13 Dec 2022 20:53


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