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Immunomagnetic t-lymphocyte depletion (ITLD) of rat bone marrow using OX-19 monoclonal antibody

Hoffman, AL and Makowka, L and Cramer, DV and Cai, X and Pascualone, A and Banner, BF and Sheahan, DG and Rettinger, P and Starzl, TE (1989) Immunomagnetic t-lymphocyte depletion (ITLD) of rat bone marrow using OX-19 monoclonal antibody. Journal of Investigative Surgery, 2 (3). 241 - 251. ISSN 0894-1939

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Abstract

Graft versus host disease (GVHD) may be abrogated and host survival prolonged by in vitro depletion of T lymphocytes from bone marrow (BM) prior to allotransplantation. Using a mouse anti-rat pan T-lymphocyte monoclonal antibody (0×19) bound to monosized, magnetic, polymer beads, T lymphocytes were removed in vitro from normal bone marrow. The removal of the T lymphocytes was confirmed by flow cytometry. Injection of the T-lymphocyte-depleted bone marrow into fully allogeneic rats prevents the induction of GVHD and prolongs host survival. A highly efficient technique of T-lymphocyte depletion using rat bone marrow is described. It involves the binding of OX-19, a MoAb directed against all rat thy-mocytes and mature peripheral T lymphocytes, to monosized, magnetic polymer spheres. Magnetic separation of T lymphocytes after mixing the allogeneic bone marrow with the bead/OX-19 complex provides for a simple, rapid depletion of T lymphocytes from the bone marrow. In vitro studies using flow cytometry and the prevention of GVHD in a fully allogeneic rat bone marrow model have been used to demonstrate the effectiveness of the depletion procedure. © 1989 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Hoffman, AL
Makowka, L
Cramer, DV
Cai, X
Pascualone, A
Banner, BF
Sheahan, DG
Rettinger, P
Starzl, TEtes11@pitt.eduTES11
Centers: Other Centers, Institutes, or Units > Thomas E. Starzl Transplantation Institute
Date: 1 January 1989
Date Type: Publication
Journal or Publication Title: Journal of Investigative Surgery
Volume: 2
Number: 3
Page Range: 241 - 251
DOI or Unique Handle: 10.3109/08941938909057430
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0894-1939
Other ID: uls-drl:31735062110675, Starzl CV No. 1026
Date Deposited: 08 Apr 2010 17:17
Last Modified: 13 Oct 2017 23:01
URI: http://d-scholarship.pitt.edu/id/eprint/4412

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