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Immunodepletion in xenotransplantation

Shapiro, R and Tzakis, AG and Scantlebury, V and Makowka, L and Watt, R and Oks, A and Yanaga, K and Podesta, L and Casavilla, A and Wos, S and Murray, J and Oral, A and D'andrea, P and Banner, B and Starzl, TE (1990) Immunodepletion in xenotransplantation. Journal of Investigative Surgery, 3 (1). 39 - 49. ISSN 0894-1939

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Abstract

Xenograft transplantation is perhaps the most immunologically difficult problem in transplantation today. An overwhelming hyperacute rejection reaction (HAR) occurs within minutes of organ implantation. Preformed antibodies are thought to initiate this process. We used a pig-to-dog renal xenograft transplant model and investigated methods of decreasing the severity of hyperacute rejection. Female pigs weighing 15-20 kg were used as donors. Recipients were mongrel dogs weighing 15-25 kg. Experimental dogs were all given a number of treatments of IgG depletion using an antibody removal system (Dupont-Excorim). This machine immunoadsorbs plasma against a column containing immobilized staphylococcal protein A, which is known to bind the IgG Fc receptor. An 84% reduction in the IgG levels and a 71% reduction in IgM levels was achieved. Postoperative assessment was made of urine output, time to onset of HAR, and histopathological examination of the rejected kidneys. Although cross-matches between donor lymphocytes and recipient sera remained strongly positive in the treated dogs, there was a two- to fourfold reduction in the titers. The time to onset of HAR was prolonged in the experimental group, and the urine output was increased slightly. The histopathologic changes in the experimental group generally showed signs of HAR, but of less intensity than in the nonimmunodepleted control group. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Shapiro, R
Tzakis, AG
Scantlebury, V
Makowka, L
Watt, R
Oks, A
Yanaga, K
Podesta, L
Casavilla, A
Wos, S
Murray, J
Oral, A
D'andrea, P
Banner, B
Starzl, TEtes11@pitt.eduTES11
Centers: Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute
Date: 1 January 1990
Date Type: Publication
Journal or Publication Title: Journal of Investigative Surgery
Volume: 3
Number: 1
Page Range: 39 - 49
DOI or Unique Handle: 10.3109/08941939009140335
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0894-1939
Other ID: uls-drl:31735062110840, Starzl CV No. 1042
Date Deposited: 08 Apr 2010 17:17
Last Modified: 14 Oct 2017 11:55
URI: http://d-scholarship.pitt.edu/id/eprint/4428

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