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The Impact of CD40L on Mature Dendritic Cell and Their EV Production

McNulty, Joseph (2023) The Impact of CD40L on Mature Dendritic Cell and Their EV Production. Master's Thesis, University of Pittsburgh. (Unpublished)

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As HIV becomes a more chronic and manageable condition, the research of the disease has shifted. ART has played a large role in raising the life expectancy of people living with HIV. However, it has some drawbacks and has created a landscape for the need of a novel and more effective therapeutic to eradicate HIV. The dendritic cell (DC) shows promise as a role player in this novel therapeutic due to its ability to link both the innate and adaptive arm of the immune systems. Several studies have shown that type-1 polarized DCs (DC1s) are more effective at driving latency reversal and CTL responses, when stimulated with CD40L, a costimulatory molecule derived from CD4+ T helper cells that has many immunologic functions. A clinically relevant type-1 polarized DC vaccine platform called the alpha-DC1 (αDC1) developed at the University of Pittsburgh is currently being studied as an alternative to more commonly used DC-based vaccines, sometimes referred to as DC2. To accurately compare the two DC subsets and to gain a better understanding of each cell type and their interaction with other immune cells, we performed tests to detail the characterization of these DC-based vaccine platforms and their responsiveness to the T helper cell signal CD40L. We assess their differences through characterization of their phenotype, morphology, cytokine production, single cell transcriptomics, and extracellular vesicle production. It is understood that the knowledge obtained from this study would give researchers an in-depth understanding of these clinically relevant DC subsets so that they may be used more effectively as an HIV therapeutic.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
McNulty, Josephjom261@pitt.edujom261
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairMailliard, Robbierbm19@pitt.edurbm19
Committee MemberMattila, Joshuajmattila@pitt.edujmattila
Committee MemberMorelli, Adrianmorelli@pitt.edumorelli
Date: 17 May 2023
Date Type: Publication
Defense Date: 20 April 2023
Approval Date: 17 May 2023
Submission Date: 29 April 2023
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Number of Pages: 67
Institution: University of Pittsburgh
Schools and Programs: School of Public Health > Infectious Diseases and Microbiology
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: N/A
Date Deposited: 17 May 2023 16:53
Last Modified: 17 May 2023 16:53


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