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Functional characteristics of lymphocytes propagated from a human multivisceral allograft.

Trager, Jonathan DK and Zeevi, Adriana and Jaffe, Ronald and Rowe, Marc I and Todo, Satoru and Starzl, Thomas E and Duquesnoy, Rene J (1990) Functional characteristics of lymphocytes propagated from a human multivisceral allograft. Clin Transplant, 4. 265 - 268.

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We investigated the characteristics of lymphocytes propagated from biopsies of the mesenteric lymph nodes, liver, and ileum of a human multivisceral allograft in order to provide functional evidence for the presence or absence of rejection and graft-versus-host disease (GVHD). The recipient was a 39-month-old girl with secretory diarrhea due to microvillus inclusion disease and end-stage liver disease secondary to prolonged parenteral nutrition. She developed a multifocal posttransplant lymphoproliferative disorder (PTLD) and died 37 days after transplantation. Four pairs of sequential mesenteric lymph node and liver biopsies (13, 17, 24, and 33 d posttransplant) and a single ileal biopsy (31 d posttransplant) were placed in culture with recombinant interleukin-2 (rIL-2) and phytohemagglutinin (PHA). T-cell phenotyping of cultured cells showed that CD8+ cells became dominant in all three tissues. The alloreactivity of biopsy-grown cells was determined using the primed lymphocyte test (PLT) and cell-mediated lympholysis test (CML). The proliferative and/or cytolytic responses of biopsy-grown cells to donor but not recipient or third party cells provided evidence for rejection and absence of GVHD. This donor-specific alloreactivity was detected before there was histologic evidence of rejection and during the period of active lymphoproliferation. This study suggests that the functional characterization of graft-infiltrating lymphocytes is useful in defining the immunologic events following multivisceral transplantation.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Trager, Jonathan DK
Zeevi, Adriana
Jaffe, Ronald
Rowe, Marc I
Todo, Satoru
Starzl, Thomas Etes11@pitt.eduTES11
Duquesnoy, Rene J
Centers: Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute
Date: 1990
Date Type: Publication
Journal or Publication Title: Clin Transplant
Volume: 4
Page Range: 265 - 268
Institution: University of Pittsburgh
Refereed: Yes
Funders: NIAAA NIH HHS (A-3176)
Other ID: uls-drl:31735062116854, Starzl CV No. 1132
Date Deposited: 08 Apr 2010 17:19
Last Modified: 08 May 2018 14:55


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