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Comparative Study of Condylar Cells of the Goat TMJ

Pinero Robles, Carlos (2023) Comparative Study of Condylar Cells of the Goat TMJ. Master's Thesis, University of Pittsburgh. (Unpublished)

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The Temporomandibular Joint (TMJ) is the unique ginglymo diarthrodial synovial joint of the body, and a vital component of the stomatognathic system. Despite the limited regenerative ability of cartilage, recent studies on goats have demonstrated that the TMJ cartilage, which is histologically defined as fibrocartilage, possesses an innate regenerative ability in vivo. These studies have also determined that the fibrocartilage subchondral interface of the TMJ condyle contains a heterogeneous population that includes stem cells/progenitor cells. This study aims to compare biological activities, such as changes in cell number over time, space filling capability and osteogenic potential of 3 types of condylar cells in the mandibular condyle of goat TMJ. It aims to go further on understanding the tissue biology of the condylar compartment. This work seeks to better understand the cells involved in subchondral bone remodeling and fibrocartilage regeneration in the context of TMJ tissue engineering. Condylar cells from the surface and cartilaginous layers were compared with cells from the subchondral bone layer and bone marrow cells for different biological activities. To assess changes of cell number overtime, condylar cells were cultured in different vessels and counted after 7 days. The growth curves of condylar cells were compared. For space filling ability, the time it took the cells to occupy a void space created by a silicone block on the culture dish was measured, as well as the distance from the leading edge to the furthest cell. For osteogenic potential, cells were cultured for 21 days in media containing inorganic phosphate and stained for Alizarin red, Von Kossa and Alkaline Phosphatase.
The results showed that all condylar cells differed with respect to all biological activities. To highlight, all three types were able to deposit copious amounts of mineral. We demonstrate that the fibrocartilage and subchondral bone interface compartment of the TMJ condyles contain a heterogeneous cell population that includes progenitor cells. These populations showed different cell numbers over time, space filling activity and mineral deposition after cultured under osteogenic condition. We verified that condylar cell populations can be harnessed through tissue engineering to guide fibrocartilage and subchondral bone regeneration.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Pinero Robles, CarlosCAP215@pitt.eduCAP2150000-0002-1390-8435
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Thesis AdvisorAlmarza,
Committee MemberBeniash,
Committee MemberSyed-Picard,
Date: 11 August 2023
Date Type: Publication
Defense Date: 19 July 2023
Approval Date: 11 August 2023
Submission Date: 4 August 2023
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Number of Pages: 35
Institution: University of Pittsburgh
Schools and Programs: School of Dental Medicine > Dental Science
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: TMJ condyle, condylar, subchondral, cartilage, regeneration
Date Deposited: 11 Aug 2023 13:01
Last Modified: 11 Aug 2023 13:01


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