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A modified apparatus for dual, sterilized, isolated perfusion of the rat liver

Liu, T and Walsh, TR and Mischinger, H and Rao, PN and Chelvakumar, P and Rubin, R and Starzl, TE (1990) A modified apparatus for dual, sterilized, isolated perfusion of the rat liver. Journal of Investigative Surgery, 3 (4). 365 - 372. ISSN 0894-1939

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The isolated perfused rat liver (IPRL) has proven to be a useful model for the study of physiology and pathology of the liver. For research in nonparenchymal cell (NPC) function that includes measurement of cytokine production (eg, TNF), it is necessary to have a sterilized perfusion system. We have modified the IPRL apparatus so as to be able to perform sterile perfusions of two livers simultaneously. The perfusion apparatus is a recirculating closed system in which the oxygenator is a plastic container separated into two chambers by a fenestrated plastic wall. A disposable macropore filter functions as both a bubble trap and perfusate filter. The sterilization process is done by immersing the various components in Benz-All solution. The tubing is disinfected by irrigation with 10% Clorox followed by 0.9% sodium chloride solution. The perfusate used is filter-sterilized Krebs buffer solution containing 0.5 g Mandol/250 mL perfusate. Not only can two organs be conveniently perfused simultaneously, but the entire system can be reliably sterilized for up to 20 consecutive perfusions. Bile production is higher and more stable with less leakage of intracellular enzymes. Many of the components are disposable and can be altered to suit the needs of a particular experiment. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Liu, T
Walsh, TR
Mischinger, H
Rao, PN
Chelvakumar, P
Rubin, R
Starzl, TEtes11@pitt.eduTES11
Centers: Other Centers, Institutes, Offices, or Units > Thomas E. Starzl Transplantation Institute
Date: 1 January 1990
Date Type: Publication
Journal or Publication Title: Journal of Investigative Surgery
Volume: 3
Number: 4
Page Range: 365 - 372
DOI or Unique Handle: 10.3109/08941939009140363
Institution: University of Pittsburgh
Refereed: Yes
ISSN: 0894-1939
Other ID: uls-drl:31735062116946, Starzl CV No. 1141
Date Deposited: 08 Apr 2010 17:19
Last Modified: 02 Feb 2019 13:57


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